Molecular identity of uncoupling proteins in thermogenic skunk cabbage

Yasuko Ito-Inaba, Yamato Hida, Hitoshi Mori, Takehito Inaba

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Thermogenic skunk cabbage has been reported to have two types of uncoupling protein (UCP), a typical 6-transmembrane (TM) SrUCPA and an atypical 5-TM SrUCPB. To verify further the role of SrUCPs in thermogenic skunk cabbage, we examined the molecular identity of SrUCPs in more detail. Both mRNA and genomic analyses supported the presence of SrUCPA, but not SrUCPB. Furthermore, SrUCP protein purified from spadix mitochondria was identified as SrUCPA by mass spectrometry. These results clearly indicate that SrUCPA is the major expressed UCP in skunk cabbage, and the presence of atypical SrUCPB is unlikely to be associated with thermogenesis of skunk cabbage.

Original languageEnglish
Pages (from-to)1911-1916
Number of pages6
JournalPlant and Cell Physiology
Volume49
Issue number12
DOIs
Publication statusPublished - 2008 Dec
Externally publishedYes

Keywords

  • mRNA splicing
  • Plant mitochondria
  • Reproductive organ
  • Skunk cabbage
  • Thermogenesis
  • Uncoupling protein

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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