Molecular identification of Fasciola spp. (Digenea: Platyhelminthes) in cattle from Vietnam

S. Nguyen, S. Amer, M. Ichikawa, T. Itagaki, Y. Fukuda, Y. Nakai

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Fasciola spp. were collected from naturally infected cattle at a local abattoir of Khanh Hoa province, Vietnam, for morphological and genetic investigations. Microscopic examination detected no sperm cells in the seminal vesicles, suggesting a parthenogenetic reproduction of the flukes. Analyses of sequences from the first and second internal transcribed spacers (ITS1 and ITS2) of the ribosomal RNA revealed that 13 out of 16 isolates were of Fasciola gigantica type, whereas three isolates presented a hybrid sequence from F. gigantica and Fasciola hepatica. Interestingly, all the mitochondrial sequences (partial COI and NDI) were of F. gigantica type, suggesting that the maternal lineage of the hybrid form is from F. gigantica. No intra-sequence variation was detected.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalParasite
Volume19
Issue number1
DOIs
Publication statusPublished - 2012 Feb

Keywords

  • COI
  • Fasciola gigantica
  • Fasciola hepatica
  • Hybridization
  • ITS
  • NDI
  • Parthenogenesis
  • Vietnam

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases

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