Molecular identification of a rat novel organic anion transporter moat1, which transports prostaglandin D2, leukotriene C4, and taurocholate

Toshiyuki Nishio, Hisanobu Adachi, Rie Nakagomi, Taro Tokui, Eiichi Sato, Masayuki Tanemoto, Kho Fujiwara, Mitsunori Okabe, Tohru Onogawa, Takehiro Suzuki, Daisuke Nakai, Kenici Shiiba, Masanori Suzuki, Haruo Ohtani, Yoshiaki Kondo, Michiaki Unno, Sadayoshi Ito, Kazuie Iinuma, Kazuo Nunoki, Seiki MatsunoTakaaki Abe

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67 Citations (Scopus)

Abstract

We have isolated a rat novel multispecific organic anion transporter, moat1. The isolated clones were originated by alternative splicing of the moat1 mRNA. The nucleotide sequences predict a protein of 682 amino acids with moderate sequence similarity to LST-1, the oatp family, and the prostaglandin transporter. Northern blot analysis of rat moat1 identified a predominant transcript of 4.4 kilonucleotides in all tissues. Northern blot and in situ hybridization analyses of rat brain further indicated that moat1 mRNA is widely distributed in neuronal cells of the central nervous system, especially in the hippocampus and cerebellum. moat1 transports prostaglandin D2 (K(m); 35.5 nM), leukotriene C4 (K(m); 3.2 μM) and taurocholate (K(m); 17.6 μM) in a sodium-independent manner. moat1 also transports prostaglandin E1, E2, thromboxane B2, and iloprost but not dehydroepiandrosterone sulfate and digoxin, of which the substrate specificity is similar, but definitively different from those of any other organic anion transporters. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)831-838
Number of pages8
JournalBiochemical and biophysical research communications
Volume275
Issue number3
DOIs
Publication statusPublished - 2000 Sep 7

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Nishio, T., Adachi, H., Nakagomi, R., Tokui, T., Sato, E., Tanemoto, M., Fujiwara, K., Okabe, M., Onogawa, T., Suzuki, T., Nakai, D., Shiiba, K., Suzuki, M., Ohtani, H., Kondo, Y., Unno, M., Ito, S., Iinuma, K., Nunoki, K., ... Abe, T. (2000). Molecular identification of a rat novel organic anion transporter moat1, which transports prostaglandin D2, leukotriene C4, and taurocholate. Biochemical and biophysical research communications, 275(3), 831-838. https://doi.org/10.1006/bbrc.2000.3377