Molecular heterogeneity of the cDNA encoding a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A

Osamu Tanabe, Gloria A. Gomez, Yasumasa Nishito, Hirofumi Usui, Masao Takeda

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Two cDNAs for possible splicing variants of a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A, were isolated. These variants were identified from human cerebral cortex by library screening and PCR, and designated δ1 and δ3 isoforms, while the previously reported isoform [Tanabe et al. (1996) FEBS Lett. 379, 107-111] was designated δ2. Compared with the δ2 isoform, the δ1 isoform contained a 32-residue insertion beginning at residue δ4, and consisted of 602 amino acids in all. The δ3 isoform lacked a 74-residue sequence corresponding to residues 1083 of the δ2 isoform, and consisted of 496 amino acids. Using isoform-specific antipeptide antisera, the 74-kDa subunit (B″ or δ) originally purified from human erythrocytes was identified as the δ1 isoform.

Original languageEnglish
Pages (from-to)52-56
Number of pages5
JournalFEBS Letters
Volume408
Issue number1
DOIs
Publication statusPublished - 1997

Keywords

  • 74-kDa regulatory subunit (B″ or δ)
  • B′
  • Human cerebral cortex
  • Human erythrocyte
  • Protein phosphatase 2A
  • Splicing variant
  • Subunit

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint

Dive into the research topics of 'Molecular heterogeneity of the cDNA encoding a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A'. Together they form a unique fingerprint.

Cite this