Molecular evidence that most RNAs required for germination and pollen tube growth are stored in the mature pollen grain in petunia

Takeshi Ishimizu, Hiroaki Kodama, Toshio Ando, Masao Watanabe

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

After landing on the stigma, the pollen grain germinates and elongates a tube to deliver its generative nuclei to the egg cell of the ovule. The molecular mechanisms involved in the drastic morphological changes in the pollen grain during this fertilization process remain largely unknown. In this study, the expression of 732 randomly selected genes in petunia pollen and pollen tubes was analyzed by microarray and quantitative PCR analyses. We found no evidence for up- regulation of any of these genes in the pollen tube. Our findings provide support at the gene level for the longstanding hypothesis that pollen germination and tube growth are not dependent on new RNA synthesis and that the large number of RNAs required for germination and tube growth are stored in mature pollen grains.

Original languageEnglish
Pages (from-to)259-263
Number of pages5
JournalGenes and Genetic Systems
Volume85
Issue number4
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Anther
  • Gene expression
  • Petunia
  • Pollen
  • Pollen tube

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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