TY - JOUR
T1 - Molecular diversity of eukaryotes in municipal wastewater treatment processes as revealed by 18S rRNA gene analysis
AU - Matsunaga, Kengo
AU - Kubota, Kengo
AU - Harada, Hideki
N1 - Publisher Copyright:
© 2014, Japanese Society of Microbial Ecology. All rights received.
PY - 2014/12/19
Y1 - 2014/12/19
N2 - Eukaryotic communities involved in sewage treatment processes have been investigated by morphological identification, but have not yet been well-characterized using molecular approaches. In the present study, eukaryotic communities were characterized by constructing 18S rRNA gene clone libraries. The phylogenetic affiliations of a total of 843 clones were Alveolata, Fungi, Rhizaria, Euglenozoa, Stramenopiles, Amoebozoa, and Viridiplantae as protozoans and Rotifera, Gastrotricha, and Nematoda as metazoans. Sixty percent of the clones had <97% sequence identity to described eukaryotes, indicating the greater diversity of eukaryotes than previously recognized. A core OTU closely related to Epistylis chrysemydis was identified, and several OTUs were shared by 4–8 libraries. Members of the uncultured lineage LKM11 in Cryptomycota were predominant fungi in sewage treatment processes. This comparative study represents an initial step in furthering understanding of the diversity and role of eukaryotes in sewage treatment processes.
AB - Eukaryotic communities involved in sewage treatment processes have been investigated by morphological identification, but have not yet been well-characterized using molecular approaches. In the present study, eukaryotic communities were characterized by constructing 18S rRNA gene clone libraries. The phylogenetic affiliations of a total of 843 clones were Alveolata, Fungi, Rhizaria, Euglenozoa, Stramenopiles, Amoebozoa, and Viridiplantae as protozoans and Rotifera, Gastrotricha, and Nematoda as metazoans. Sixty percent of the clones had <97% sequence identity to described eukaryotes, indicating the greater diversity of eukaryotes than previously recognized. A core OTU closely related to Epistylis chrysemydis was identified, and several OTUs were shared by 4–8 libraries. Members of the uncultured lineage LKM11 in Cryptomycota were predominant fungi in sewage treatment processes. This comparative study represents an initial step in furthering understanding of the diversity and role of eukaryotes in sewage treatment processes.
KW - 18S rRNA gene
KW - Alveolata
KW - Eukaryotic community composition
KW - Fungi
KW - Municipal wastewater treatment
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U2 - 10.1264/jsme2.ME14112
DO - 10.1264/jsme2.ME14112
M3 - Article
C2 - 25491751
AN - SCOPUS:84919417806
VL - 29
SP - 401
EP - 407
JO - Microbes and Environments
JF - Microbes and Environments
SN - 1342-6311
IS - 4
ER -