Molecular diversity of eukaryotes in municipal wastewater treatment processes as revealed by 18S rRNA gene analysis

Kengo Matsunaga, Kengo Kubota, Hideki Harada

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Eukaryotic communities involved in sewage treatment processes have been investigated by morphological identification, but have not yet been well-characterized using molecular approaches. In the present study, eukaryotic communities were characterized by constructing 18S rRNA gene clone libraries. The phylogenetic affiliations of a total of 843 clones were Alveolata, Fungi, Rhizaria, Euglenozoa, Stramenopiles, Amoebozoa, and Viridiplantae as protozoans and Rotifera, Gastrotricha, and Nematoda as metazoans. Sixty percent of the clones had <97% sequence identity to described eukaryotes, indicating the greater diversity of eukaryotes than previously recognized. A core OTU closely related to Epistylis chrysemydis was identified, and several OTUs were shared by 4–8 libraries. Members of the uncultured lineage LKM11 in Cryptomycota were predominant fungi in sewage treatment processes. This comparative study represents an initial step in furthering understanding of the diversity and role of eukaryotes in sewage treatment processes.

Original languageEnglish
Pages (from-to)401-407
Number of pages7
JournalMicrobes and environments
Volume29
Issue number4
DOIs
Publication statusPublished - 2014 Dec 19

Keywords

  • 18S rRNA gene
  • Alveolata
  • Eukaryotic community composition
  • Fungi
  • Municipal wastewater treatment

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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