Molecular dissection of α- and dense-core granule secretion of platelets

Akira Yoshioka; Hisanori Horiuchi; Ryutaro Shirakawa; Hiroaki Nishioka; Arata Tabuchi; Tomohito Higashi; Akitsugu Yamamoto; Toru Kita

doi:10.1111/j.1749-6632.2001.tb03973.x

Molecular dissection of α- and dense-core granule secretion of platelets

Akira Yoshioka, Hisanori Horiuchi, Ryutaro Shirakawa, Hiroaki Nishioka, Arata Tabuchi, Tomohito Higashi, Akitsugu Yamamoto, Toru Kita

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Upon activation, platelets release many active substances stored in α- and dense-core granules. However, the molecular mechanisms governing the regulated exocytosis are not yet fully understood. We have established an assay system using streptolysin-O-permeabilized platelets to analyze the Ca²⁺-induced secretions of von Willebrand factor stored in α-granules and [³H]5-hydroxytryptamine (5-HT) in dense-core granules. Using the assay, we found that small GTPase Rab4 regulates α-, but not dense-core, granule secretion in platelets. Furthermore, we purified a cytosolic essential protein and currently are analyzing its function.

Original language	English
Pages (from-to)	403-406
Number of pages	4
Journal	Annals of the New York Academy of Sciences
Volume	947
DOIs	https://doi.org/10.1111/j.1749-6632.2001.tb03973.x
Publication status	Published - 2001
Externally published	Yes

Keywords

Dense-core granules
Molecular dissection
Platelet secretion
α-granules

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
History and Philosophy of Science

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10.1111/j.1749-6632.2001.tb03973.x

Cite this

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title = "Molecular dissection of α- and dense-core granule secretion of platelets",

abstract = "Upon activation, platelets release many active substances stored in α- and dense-core granules. However, the molecular mechanisms governing the regulated exocytosis are not yet fully understood. We have established an assay system using streptolysin-O-permeabilized platelets to analyze the Ca2+-induced secretions of von Willebrand factor stored in α-granules and [3H]5-hydroxytryptamine (5-HT) in dense-core granules. Using the assay, we found that small GTPase Rab4 regulates α-, but not dense-core, granule secretion in platelets. Furthermore, we purified a cytosolic essential protein and currently are analyzing its function.",

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language = "English",

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journal = "Annals of the New York Academy of Sciences",

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TY - JOUR

T1 - Molecular dissection of α- and dense-core granule secretion of platelets

AU - Yoshioka, Akira

AU - Horiuchi, Hisanori

AU - Shirakawa, Ryutaro

AU - Nishioka, Hiroaki

AU - Tabuchi, Arata

AU - Higashi, Tomohito

AU - Yamamoto, Akitsugu

AU - Kita, Toru

PY - 2001

Y1 - 2001

N2 - Upon activation, platelets release many active substances stored in α- and dense-core granules. However, the molecular mechanisms governing the regulated exocytosis are not yet fully understood. We have established an assay system using streptolysin-O-permeabilized platelets to analyze the Ca2+-induced secretions of von Willebrand factor stored in α-granules and [3H]5-hydroxytryptamine (5-HT) in dense-core granules. Using the assay, we found that small GTPase Rab4 regulates α-, but not dense-core, granule secretion in platelets. Furthermore, we purified a cytosolic essential protein and currently are analyzing its function.

AB - Upon activation, platelets release many active substances stored in α- and dense-core granules. However, the molecular mechanisms governing the regulated exocytosis are not yet fully understood. We have established an assay system using streptolysin-O-permeabilized platelets to analyze the Ca2+-induced secretions of von Willebrand factor stored in α-granules and [3H]5-hydroxytryptamine (5-HT) in dense-core granules. Using the assay, we found that small GTPase Rab4 regulates α-, but not dense-core, granule secretion in platelets. Furthermore, we purified a cytosolic essential protein and currently are analyzing its function.

KW - Dense-core granules

KW - Molecular dissection

KW - Platelet secretion

KW - α-granules

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JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

SN - 0077-8923

ER -

Molecular dissection of α- and dense-core granule secretion of platelets

Abstract

Keywords

ASJC Scopus subject areas

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