Abstract
Upon activation, platelets release many active substances stored in α- and dense-core granules. However, the molecular mechanisms governing the regulated exocytosis are not yet fully understood. We have established an assay system using streptolysin-O-permeabilized platelets to analyze the Ca2+-induced secretions of von Willebrand factor stored in α-granules and [3H]5-hydroxytryptamine (5-HT) in dense-core granules. Using the assay, we found that small GTPase Rab4 regulates α-, but not dense-core, granule secretion in platelets. Furthermore, we purified a cytosolic essential protein and currently are analyzing its function.
Original language | English |
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Pages (from-to) | 403-406 |
Number of pages | 4 |
Journal | Annals of the New York Academy of Sciences |
Volume | 947 |
DOIs | |
Publication status | Published - 2001 |
Externally published | Yes |
Keywords
- Dense-core granules
- Molecular dissection
- Platelet secretion
- α-granules
ASJC Scopus subject areas
- Neuroscience(all)
- Biochemistry, Genetics and Molecular Biology(all)
- History and Philosophy of Science