TY - JOUR
T1 - Molecular detection and direct enumeration of methanogenic Archaea and methanotrophic Bacteria in domestic solid waste landfill soils
AU - Chen, Ann Cheng
AU - Ueda, Kumiko
AU - Sekiguchi, Yuji
AU - Ohashi, Akiyoshi
AU - Harada, Hideki
PY - 2003/9
Y1 - 2003/9
N2 - Methane oxidizing and producing activities of cover soil (10, 30 cm depth) and burial waste (1, 3 m depth) were evaluated: top cover soil (10 cm) had the highest methane oxidizing activity, while 1 m depth buried waste showed the highest methane producing potential. All the sequences of the 1 m sample were found to be closely related to 16S rDNAs of mainly hydrogenotrophic methanogens known, such as genera Methanosarcina, Methanoculleus, and Methanobacterium. We developed a modified fluorescence in situ hybridization (FISH) direct counting method for landfill samples, resulting in the detection of approx. 1% of total cells as archaeal cells (presumably methanogens). However, probe-positive cells could not be found with probes for methanotrophs by the methods.
AB - Methane oxidizing and producing activities of cover soil (10, 30 cm depth) and burial waste (1, 3 m depth) were evaluated: top cover soil (10 cm) had the highest methane oxidizing activity, while 1 m depth buried waste showed the highest methane producing potential. All the sequences of the 1 m sample were found to be closely related to 16S rDNAs of mainly hydrogenotrophic methanogens known, such as genera Methanosarcina, Methanoculleus, and Methanobacterium. We developed a modified fluorescence in situ hybridization (FISH) direct counting method for landfill samples, resulting in the detection of approx. 1% of total cells as archaeal cells (presumably methanogens). However, probe-positive cells could not be found with probes for methanotrophs by the methods.
KW - Archaea
KW - Enumeration
KW - Fluorescence in situ hybridization
KW - Landfill
KW - Methanotrophs
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U2 - 10.1023/A:1025461915495
DO - 10.1023/A:1025461915495
M3 - Article
C2 - 14571983
AN - SCOPUS:0141565120
VL - 25
SP - 1563
EP - 1569
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 18
ER -