Molecular detection and direct enumeration of methanogenic Archaea and methanotrophic Bacteria in domestic solid waste landfill soils

Ann Cheng Chen, Kumiko Ueda, Yuji Sekiguchi, Akiyoshi Ohashi, Hideki Harada

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Methane oxidizing and producing activities of cover soil (10, 30 cm depth) and burial waste (1, 3 m depth) were evaluated: top cover soil (10 cm) had the highest methane oxidizing activity, while 1 m depth buried waste showed the highest methane producing potential. All the sequences of the 1 m sample were found to be closely related to 16S rDNAs of mainly hydrogenotrophic methanogens known, such as genera Methanosarcina, Methanoculleus, and Methanobacterium. We developed a modified fluorescence in situ hybridization (FISH) direct counting method for landfill samples, resulting in the detection of approx. 1% of total cells as archaeal cells (presumably methanogens). However, probe-positive cells could not be found with probes for methanotrophs by the methods.

Original languageEnglish
Pages (from-to)1563-1569
Number of pages7
JournalBiotechnology Letters
Volume25
Issue number18
DOIs
Publication statusPublished - 2003 Sep 1

Keywords

  • Archaea
  • Enumeration
  • Fluorescence in situ hybridization
  • Landfill
  • Methanotrophs

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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