Molecular cloning, tissue expression, and subcellular localization of porcine peptidoglycan recognition proteins 3 and 4

Wataru Ueda, Masanori Tohno, Tomoyuki Shimazu, Hitomi Fujie, Hisashi Aso, Yasushi Kawai, Muneo Numasaki, Tadao Saito, Haruki Kitazawa

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Peptidoglycan recognition proteins (PGRPs) are innate immune molecules that are present in most invertebrates and vertebrates. Mammals have four PGRPs, PGLYRP1-4. In the present study, we cloned the cDNAs encoding porcine PGLYRP3 and 4 from the esophagus of adult swine. The length of the complete open reading frames of porcine PGLYRP3 and 4 are identical and contain 1125. bp encoding 374 amino acid residues. The amino acid sequences of these two proteins were more similar to their human orthologs (78.9% [PGLYRP3] and 73.9% [PGLYRP4]) than to their mouse orthologs (71.3% [PGLYRP3] and 67.9% [PGLYRP4]). Expression analysis revealed that both PGLYRP3 and 4 were more strongly expressed in digestive tract, especially the esophagus, than in immune organs such as spleen or mesenteric lymph nodes in both newborn and adult swine. To analyze the subcellular distribution of porcine PGLYRP1-4, we constructed transfectant cell lines. Western blot and flow cytometric analyses revealed that porcine PGLYRP3 and 4 are not only secreted, but also expressed on the cell surface, unlike PGLYRP1 and 2. These results should help contribute to the understanding of PGLYRP3- and 4-mediated immune responses via their recognition of intestinal microorganisms in newborn and adult swine.

Original languageEnglish
Pages (from-to)148-154
Number of pages7
JournalVeterinary Immunology and Immunopathology
Volume143
Issue number1-2
DOIs
Publication statusPublished - 2011 Sep 15

Keywords

  • CDNA cloning
  • Expression analysis
  • PGLYRP4
  • Peptidoglycan recognition protein (PGLYRP) 3
  • Porcine

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

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