TY - JOUR
T1 - Molecular cloning, tissue expression, and subcellular localization of porcine peptidoglycan recognition proteins 3 and 4
AU - Ueda, Wataru
AU - Tohno, Masanori
AU - Shimazu, Tomoyuki
AU - Fujie, Hitomi
AU - Aso, Hisashi
AU - Kawai, Yasushi
AU - Numasaki, Muneo
AU - Saito, Tadao
AU - Kitazawa, Haruki
N1 - Funding Information:
This study was partly supported by a Grant-in-Aid for Scientific Research (B) (2) (No. 21380164 ) and Challenging Exploratory Research (No. 20658061 ) to H. Kitazawa, and Young Scientists (Start-up) (No. 21880053 ) to M. Tohno from the Japan Society for the Promotion of Science .
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/9/15
Y1 - 2011/9/15
N2 - Peptidoglycan recognition proteins (PGRPs) are innate immune molecules that are present in most invertebrates and vertebrates. Mammals have four PGRPs, PGLYRP1-4. In the present study, we cloned the cDNAs encoding porcine PGLYRP3 and 4 from the esophagus of adult swine. The length of the complete open reading frames of porcine PGLYRP3 and 4 are identical and contain 1125. bp encoding 374 amino acid residues. The amino acid sequences of these two proteins were more similar to their human orthologs (78.9% [PGLYRP3] and 73.9% [PGLYRP4]) than to their mouse orthologs (71.3% [PGLYRP3] and 67.9% [PGLYRP4]). Expression analysis revealed that both PGLYRP3 and 4 were more strongly expressed in digestive tract, especially the esophagus, than in immune organs such as spleen or mesenteric lymph nodes in both newborn and adult swine. To analyze the subcellular distribution of porcine PGLYRP1-4, we constructed transfectant cell lines. Western blot and flow cytometric analyses revealed that porcine PGLYRP3 and 4 are not only secreted, but also expressed on the cell surface, unlike PGLYRP1 and 2. These results should help contribute to the understanding of PGLYRP3- and 4-mediated immune responses via their recognition of intestinal microorganisms in newborn and adult swine.
AB - Peptidoglycan recognition proteins (PGRPs) are innate immune molecules that are present in most invertebrates and vertebrates. Mammals have four PGRPs, PGLYRP1-4. In the present study, we cloned the cDNAs encoding porcine PGLYRP3 and 4 from the esophagus of adult swine. The length of the complete open reading frames of porcine PGLYRP3 and 4 are identical and contain 1125. bp encoding 374 amino acid residues. The amino acid sequences of these two proteins were more similar to their human orthologs (78.9% [PGLYRP3] and 73.9% [PGLYRP4]) than to their mouse orthologs (71.3% [PGLYRP3] and 67.9% [PGLYRP4]). Expression analysis revealed that both PGLYRP3 and 4 were more strongly expressed in digestive tract, especially the esophagus, than in immune organs such as spleen or mesenteric lymph nodes in both newborn and adult swine. To analyze the subcellular distribution of porcine PGLYRP1-4, we constructed transfectant cell lines. Western blot and flow cytometric analyses revealed that porcine PGLYRP3 and 4 are not only secreted, but also expressed on the cell surface, unlike PGLYRP1 and 2. These results should help contribute to the understanding of PGLYRP3- and 4-mediated immune responses via their recognition of intestinal microorganisms in newborn and adult swine.
KW - CDNA cloning
KW - Expression analysis
KW - PGLYRP4
KW - Peptidoglycan recognition protein (PGLYRP) 3
KW - Porcine
UR - http://www.scopus.com/inward/record.url?scp=79961027787&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79961027787&partnerID=8YFLogxK
U2 - 10.1016/j.vetimm.2011.05.026
DO - 10.1016/j.vetimm.2011.05.026
M3 - Article
C2 - 21665294
AN - SCOPUS:79961027787
VL - 143
SP - 148
EP - 154
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
SN - 0165-2427
IS - 1-2
ER -