Molecular cloning of the nemA gene encoding N-ethylmaleimide reductase from Escherichia coli

Koichi Miura, Yoshihisa Tomioka, Hideaki Suzuki, Motoko Yonezawa, Takanori Hishinuma, Michinao Mizugaki

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

Using the gene mapping membrane technique, we identified a gene (nemA) that encodes N-ethylmaleimide reductase in Escherichia coli. The open reading frame encodes a polypeptide of 365 amino acids with a molecular mass of 39,514 Da. The deduced amino acid sequence showed a high degree of homology (87% identical) with the pentaerythritol tetranitrate reductase of Enterobacter cloacae and the morphinone reductase of Pseudomonasputida (52% identical).

Original languageEnglish
Pages (from-to)110-112
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume20
Issue number1
DOIs
Publication statusPublished - 1997 Jan

Keywords

  • Escherichia coli
  • N-ethylmaleimide reductase
  • Old Yellow Enzyme
  • unsaturated fatty acid

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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