Molecular cloning of the human ah receptor gene promoter

Hidetaka Eguchi, Shin Ichi Hayashi, Junko Watanabe, Osamu Gotoh, Kanamc Kawajiri

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A λ phage clone containing a promoter region of the human Ah receptor gene was isolated. This clone spanned 13.8 kb and contained the 1st exon, the sequence of which completely matched the reported Ah receptor cDNA. Using RNase protection assay and primer extension analysis, the transcription initiation sites were determined to be 643 and 615 bp upstream of the translational initiation codon ATG. This promoter did not contain a TATA box, while multiple GC boxes were present close to the determined transcription initiation sites. Comparison of the 5’ flank sequence of the human Ah receptor with its murine equivalent showed several well conserved regions, containing binding sites for known transcription factors, such as Spl. The promoter activity was confirmed by transient transfection of chimeric constructs of the Ah receptor gene and reporter gene luciferase into hepatoma HepG2 cells.

Original languageEnglish
Pages (from-to)615-622
Number of pages8
JournalBiochemical and biophysical research communications
Volume203
Issue number1
DOIs
Publication statusPublished - 1994 Aug 30
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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