TY - JOUR
T1 - Molecular Cloning of the Glucoamylase Gene of Aspergillus shirousami and Its Expression in Aspergillus Oryzae
AU - Shibuya, Ichiro
AU - Tamura, Gakuzo
AU - Gomi, Katsuya
AU - Iimura, Yuzuru
AU - Takahashi, Kojiro
AU - Hara, Shodo
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1990
Y1 - 1990
N2 - The glucoamylase enzyme (GAase) gene from Aspergillus shirousami was cloned and sequenced from genomic and cDNA libraries. The genomic gene was located in the 5.4 kb EcoRI fragment. The deduced amino acid sequence of GAase contained 639 amino acid residues with a relative molecular mass of approximately 68,000 daltons (non-glycosylated form). The genomic gene of A. shirousami GAase was introduced into Aspergillus oryzae. These transformants had increased GAase and raw starch degradation (RSD) activity in culture media and in rice-koji extracts. Analysis by Southern, Northern, SDS-PAGE, and Western blot techniques confirmed the foreign gene was correctly transcribed, translated, and expressed in A. oryzae.
AB - The glucoamylase enzyme (GAase) gene from Aspergillus shirousami was cloned and sequenced from genomic and cDNA libraries. The genomic gene was located in the 5.4 kb EcoRI fragment. The deduced amino acid sequence of GAase contained 639 amino acid residues with a relative molecular mass of approximately 68,000 daltons (non-glycosylated form). The genomic gene of A. shirousami GAase was introduced into Aspergillus oryzae. These transformants had increased GAase and raw starch degradation (RSD) activity in culture media and in rice-koji extracts. Analysis by Southern, Northern, SDS-PAGE, and Western blot techniques confirmed the foreign gene was correctly transcribed, translated, and expressed in A. oryzae.
UR - http://www.scopus.com/inward/record.url?scp=0025473723&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025473723&partnerID=8YFLogxK
U2 - 10.1271/bbb1961.54.1905
DO - 10.1271/bbb1961.54.1905
M3 - Article
C2 - 1368603
AN - SCOPUS:0025473723
VL - 54
SP - 1905
EP - 1914
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 8
ER -