Molecular cloning of the defense factor in the albumen gland of the sea hare Aplysia kurodai

Nobuhiko Takamatsu, Tadayoshi Shiba, Koji Muramoto, Hisao Kamiya

    Research output: Contribution to journalArticlepeer-review

    37 Citations (Scopus)

    Abstract

    Aplysianin-A, an antibacterial glycoprotein in the albumen gland of the sea hare Aplysia kurodai, inhibited the growth of both Gram-positive and Gram-negative bacteria. Aplysianin-A cDNA clones were isolated from an albumen gland cDNA library. Sequence analysis reveals that aplysianin-A is produced as a precursor protein of 556 amino acid residues with a signal peptide of 19 amino acid residues and contains 6 potential N-glycosylation sites. Aplysianin-A mRNA was expressed tissue-specifically in the albumen gland. Homology search reveals that aplysianin-A has a 50% overall amino acid sequence homology to achacin, an antibacterial glycoprotein of the giant African snail Achatina fulica.

    Original languageEnglish
    Pages (from-to)373-376
    Number of pages4
    JournalFEBS Letters
    Volume377
    Issue number3
    DOIs
    Publication statusPublished - 1995 Dec 27

    Keywords

    • Antibacterial glycoprotein
    • Defense factor
    • Sea hare
    • cDNA cloning

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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