Molecular cloning of the defense factor in the albumen gland of the sea hare Aplysia kurodai

Nobuhiko Takamatsu, Tadayoshi Shiba, Koji Muramoto, Hisao Kamiya

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Aplysianin-A, an antibacterial glycoprotein in the albumen gland of the sea hare Aplysia kurodai, inhibited the growth of both Gram-positive and Gram-negative bacteria. Aplysianin-A cDNA clones were isolated from an albumen gland cDNA library. Sequence analysis reveals that aplysianin-A is produced as a precursor protein of 556 amino acid residues with a signal peptide of 19 amino acid residues and contains 6 potential N-glycosylation sites. Aplysianin-A mRNA was expressed tissue-specifically in the albumen gland. Homology search reveals that aplysianin-A has a 50% overall amino acid sequence homology to achacin, an antibacterial glycoprotein of the giant African snail Achatina fulica.

Original languageEnglish
Pages (from-to)373-376
Number of pages4
JournalFEBS Letters
Volume377
Issue number3
DOIs
Publication statusPublished - 1995 Dec 27
Externally publishedYes

Keywords

  • Antibacterial glycoprotein
  • Defense factor
  • Sea hare
  • cDNA cloning

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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