Molecular cloning of synaphins/complexins, cytosolic proteins involved in transmitter release, in the electric organ of an electric ray (Narke japonica)

Toru Ishizuka, Hideo Saisu, Toshiharu Suzuki, Yutaka Kirino, Teruo Abe

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Synaphins/complexins are cytosolic proteins associated with the docking/fusion complex crucial to transmitter release. The electric organ of the electric ray Narke japonica contained at least two kinds of synaphins as revealed by immunoblotting. cDNAs for three synaphins were cloned from a cDNA library prepared from the electric lobe where cell bodies of electromotor nerves innervating the electric organ exist. The proteins encoded by these cDNAs were named Nj-synaphins la, lb and 2 on the basis of their high homologies (83-93%) to mammalian synaphins 1 and 2. Nj-Synaphins were immunoprecipitated by an anti-syntaxin monoclonal antibody together with syntaxin SNAP-25 and VAMP (synaptobrevin), suggesting the presence of a docking/fusion complex similar to that in the mammalian brain.

    Original languageEnglish
    Pages (from-to)107-110
    Number of pages4
    JournalNeuroscience Letters
    Volume232
    Issue number2
    DOIs
    Publication statusPublished - 1997 Aug 29

    Keywords

    • Docking/fusion complex
    • Electric organ
    • Narke japonica
    • SNAP-25
    • Synaphin/complexin
    • Syntaxin
    • Transmitter release
    • VAMP/synaptobrevin

    ASJC Scopus subject areas

    • Neuroscience(all)

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