Molecular cloning of rat cDNA for cytosolic phospholipase A2 and the increased gene expression in the dentate gyrus following transient forebrain ischemia

Yuji Owada, Teiji Tominaga, Takashi Yoshimoto, Hisatake Kondo

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)

Abstract

Rat cytosolic phospholipase A2 (cPLA2) cDNA was cloned from rat brain. The cDNA showed a high homology of 90% in the nucleotide sequence of the coding region with the human counterpart. By in situ hybridization, the gene expression for cPLA2 was detected in the hippocampus, olfactory bulb, and cerebellar granular cells at very low level of normal rats. The expression was markedly increased in the dentate granule cells of postischemic rat brain. This alteration of the gene expression was discussed in relation to the free fatty acid-mediated neurotoxicity.

Original languageEnglish
Pages (from-to)364-368
Number of pages5
JournalMolecular Brain Research
Volume25
Issue number3-4
DOIs
Publication statusPublished - 1994 Sep

Keywords

  • Ischemia
  • Phospholipase A
  • Rat brain
  • cDNA cloning

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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