TY - JOUR
T1 - Molecular cloning of members of the s-multigene family in self-incompatible brassica campestris l.
AU - Suzuki, Go
AU - Watanabe, Masao
AU - Toriyama, Kinya
AU - Isogai, Akira
AU - Hinata, Kokichi
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Special Research on Priority Areas (nos. 07281102, 07281101 and 07281103; Genetic Dissection of Sexual Differentiation and Pollination Process in Higher Plants) from the Ministry of Education, Science and Culture, Japan. We thank Yukihiro Ito and Tohru Tsuchiya, members of our laboratory, for helpful advice related to the present study.
PY - 1995/10
Y1 - 1995/10
N2 - We isolated 12 groups of genomic clones that contained SLG-homologous regions from a genomic library constructed from an S9 homozygote of self-incompatible Brassica campestris. Both SLG9 and SRK9 genomic clones, which are located within the self-incompatibility (S) locus, were included in these groups. The promoter regions of SLG9 and SRK9 were completely identical for at least 200 bp upstream from their respective initiation codons (ATG). The five sequence elements (boxes I to V) that are conserved in the promoters of SLG and SRK genes were also found in the SLG9 and SRK9 clones. However, one conserved element (box III) unexpectedly lacked 7 of 11 bp, although box III has been considered necessary for expression in pistil. The other ten groups of genomic clones were classified into six SRK-like groups and four SLG-like groups. These results indicate that SLG, SRK, SLG-like, and SRK-like genes form a large S-multigene family in B. campestris.
AB - We isolated 12 groups of genomic clones that contained SLG-homologous regions from a genomic library constructed from an S9 homozygote of self-incompatible Brassica campestris. Both SLG9 and SRK9 genomic clones, which are located within the self-incompatibility (S) locus, were included in these groups. The promoter regions of SLG9 and SRK9 were completely identical for at least 200 bp upstream from their respective initiation codons (ATG). The five sequence elements (boxes I to V) that are conserved in the promoters of SLG and SRK genes were also found in the SLG9 and SRK9 clones. However, one conserved element (box III) unexpectedly lacked 7 of 11 bp, although box III has been considered necessary for expression in pistil. The other ten groups of genomic clones were classified into six SRK-like groups and four SLG-like groups. These results indicate that SLG, SRK, SLG-like, and SRK-like genes form a large S-multigene family in B. campestris.
KW - Brassica campestris L.
KW - Genomic clones
KW - Multigene family
KW - S-locus glycoprotein
KW - S-receptor kinase
KW - Self-incompatibility
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M3 - Article
C2 - 8564299
AN - SCOPUS:0029393683
VL - 36
SP - 1273
EP - 1280
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 7
ER -