TY - JOUR
T1 - Molecular cloning of cDNAs encoding α1, α2, and β subunits of rat brain platelet-activating factor acetylhydrolase
AU - Watanabe, MacHiko
AU - Aoki, Junken
AU - Manya, Hiroshi
AU - Arai, Hiroyuki
AU - Inoue, Keizo
N1 - Funding Information:
We thank Drs. M. Mori and T. Shimizu for generous help in Northern blot analysis of primary cultured cells. This work was supported in part by research grants from the Ministry of Education, Science, Sports, and Culture of Japan, and by special coordination funds from the Science and Technology Agency of the Japanese Government.
PY - 1998/1/2
Y1 - 1998/1/2
N2 - Brain intracellular platelet-activating factor acetylhydrolase (PAF-AH(Ib)) is a tertiary G-protein-complex-like heterotrimeric enzyme which is composed of α1, α2, and β subunits and is implicated in stages of brain development such as the formation of the blain cortex. We have isolated and sequenced cDNA clones encoding these three subunits of rat brain PAF-AH(Ib). The amino acid sequences of brain PAF-AH has shown an extremely high homology among mammalian species. The tissue distribution of the three subunits was examined by Northern blot analysis. Although the mRNAs were detected in various organs, the ratio of the level of mRNA expression for the three subunits differed among rat tissues, raising tile possibility that isoform(s) other than the heterotrimeric isoform exist in certain tissues.
AB - Brain intracellular platelet-activating factor acetylhydrolase (PAF-AH(Ib)) is a tertiary G-protein-complex-like heterotrimeric enzyme which is composed of α1, α2, and β subunits and is implicated in stages of brain development such as the formation of the blain cortex. We have isolated and sequenced cDNA clones encoding these three subunits of rat brain PAF-AH(Ib). The amino acid sequences of brain PAF-AH has shown an extremely high homology among mammalian species. The tissue distribution of the three subunits was examined by Northern blot analysis. Although the mRNAs were detected in various organs, the ratio of the level of mRNA expression for the three subunits differed among rat tissues, raising tile possibility that isoform(s) other than the heterotrimeric isoform exist in certain tissues.
KW - PAF
KW - PAF acetylhydrolase
KW - Platelet-activating factor
KW - α1 Subunit
KW - α2 Subunit
KW - β Subunit
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U2 - 10.1016/S0167-4889(97)00128-6
DO - 10.1016/S0167-4889(97)00128-6
M3 - Article
C2 - 9459487
AN - SCOPUS:0032472299
VL - 1401
SP - 73
EP - 79
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
SN - 0167-4889
IS - 1
ER -