Molecular cloning of cDNA of S100α subunit mRNA

Ryozo Kuwano, Toshinaga Maeda, Hiroshi Usui, Kazuaki Araki, Toru Yamakuni, Yuka Ohshima, Tadashi Kurihara, Yasuo Takahashi

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The primary structure of the bovine S-100α mRNA on the basis of molecular cloning and sequence analysis of the cDNA are described. The sequence is composed of 532 bp which include the 282 bp of the complete coding region, 89 bp at the 5'-noncoding region, 161 bp at the 3'-noncoding region, polyadenylation signal, ATTAAA and poly(A) tail. Northern blot analysis shows that the size of S-100α mRNA is about 700-800 bases long and a single mRNA occurs in bovine brain. Bovine brain contains both S100α and β subunits and their mRNAs. In contrast, the rat brain contains only S 100α subunit and its mRNA.

Original languageEnglish
Pages (from-to)97-101
Number of pages5
JournalFEBS Letters
Volume202
Issue number1
DOIs
Publication statusPublished - 1986 Jun 23

Keywords

  • S-100 protein mRNA cDNA cloning Nudeotide sequence

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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