Molecular cloning of cDNA for the 29 kDa proteinase participating in decomposition of the larval fat body during metamorphosis of Sarcophaga peregrina (flesh fly)

Noboru Takahashi, Shoichiro Kurata, Shunji Natori

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

A cDNA clone for the 29 kDa proteinase participating in tissue disintegration during metamorphosis of Sarcophaga was isolated. This proteinase, named Sarcophaga cathepsin B, consisted of 256 amino acid residues, and contained three putative N-glycosylation sites. By comparison with other cathepsins B, its unique substrate specificity was partly explained by Ala at position 248.

Original languageEnglish
Pages (from-to)153-157
Number of pages5
JournalFEBS Letters
Volume334
Issue number2
DOIs
Publication statusPublished - 1993 Nov 15
Externally publishedYes

Keywords

  • Cathepsin B
  • Fat body decomposition
  • Metamorphosis
  • cDNA cloning

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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