TY - JOUR
T1 - Molecular cloning of cDNA for Sarcophaga prolyl endopeptidase and characterization of the recombinant enzyme produced by an E. coli expression system
AU - Ohtsuki, Sumio
AU - Homma, Ko Ichi
AU - Kurata, Shoichiro
AU - Natori, Shunji
PY - 1997/1/1
Y1 - 1997/1/1
N2 - A cDNA for prolyl endopeptidase (PEP) of Sarcophaga peregrina (flesh fly) was cloned and its sequence determined. The overall amino acid sequence identity between Sarcophaga and mammalian PEPs was 53%, indicating that these enzymes are structurally very similar. Northern blot hybridization revealed that the Sarcophaga PEP gene was activated significantly at the eversion stage of imaginal disc differentiation. We obtained recombinant PEP by expressing the cDNA in Escherichia coli. The recombinant and authentic enzymes showed almost identical characteristics, in terms of substrate specificities and sensitivities to inhibitors.
AB - A cDNA for prolyl endopeptidase (PEP) of Sarcophaga peregrina (flesh fly) was cloned and its sequence determined. The overall amino acid sequence identity between Sarcophaga and mammalian PEPs was 53%, indicating that these enzymes are structurally very similar. Northern blot hybridization revealed that the Sarcophaga PEP gene was activated significantly at the eversion stage of imaginal disc differentiation. We obtained recombinant PEP by expressing the cDNA in Escherichia coli. The recombinant and authentic enzymes showed almost identical characteristics, in terms of substrate specificities and sensitivities to inhibitors.
KW - Imaginal disc differentiation
KW - Prolyl endopeptidase
KW - Recombinant enzyme
KW - Sarcophaga peregrina
UR - http://www.scopus.com/inward/record.url?scp=0031127995&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031127995&partnerID=8YFLogxK
U2 - 10.1016/S0965-1748(97)00004-0
DO - 10.1016/S0965-1748(97)00004-0
M3 - Article
C2 - 9134713
AN - SCOPUS:0031127995
VL - 27
SP - 337
EP - 343
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
SN - 0965-1748
IS - 4
ER -