Molecular cloning of a Pseudomonas paucimobilis gene encoding a 17-kilodalton polypeptide that eliminates HCl molecules from γ-hexachlorocyclohexane

R. Imai, Y. Nagata, M. Fukuda, M. Takagi, K. Yano

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Pseudomonas paucimobilis UT26 is capable of growing on γ-hexachlorocyclohexane (γ-HCH). A genomic library of P. paucimobilis UT26 was constructed in Pseudomonas putida by using the broad-host-range cosmid vector pKS13. After 2,300 clones were screened by gas chromatography, 3 clones showing γ-HCH degradation were detected. A 5-kb fragment from one of the cosmid clones was subcloned into pUC118, and subsequent deletion and gas chromatography-mass spectrometry analyses revealed that a fragment of ca. 500 bp was responsible for the conversion of γ-HCH to 1,2,4-trichlorobenzene via γ-pentachlorocyclohexene. Nucleotide sequence analysis revealed an open reading frame (linA) of 465 bp within the fragment. The nucleotide sequence of the linA gene and the deduced amino acid sequence showed no similarity to any known sequences. The product of the linA gene was 16.5 kDa according to sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Original languageEnglish
Pages (from-to)6811-6819
Number of pages9
JournalJournal of bacteriology
Volume173
Issue number21
DOIs
Publication statusPublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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