Molecular cloning of a genomic dna for enolase from aspergillus oryzae

Masayuki Machida, Trini Violeta Jime’nez Gonzale, Lim Kong Boon, Katsuya Gomi, Yoshifumi Jigami

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

We have isolated an enolase gene (enoA) from Aspergillus oryzae by heterologous hybridization using the corresponding Saccharomyces cerevisiae ENO2 gene as a probe. A 2.9-kb BglII-fragment contained the entire structural gene enoA including 5′- and 3′- flanking regions. The homology between A. oryzae enoA and S. cerevisiae ENO2 genes is 66.9% when introns are removed. Genomic Southern analysis indicated that there is only one enolase gene in A. oryzae.

Original languageEnglish
Pages (from-to)161-163
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume60
Issue number1
DOIs
Publication statusPublished - 1996 Jan 1
Externally publishedYes

Keywords

  • Cholesterol-lowering action
  • Hypocholesterolemic action
  • Octopine
  • Opine
  • Rat

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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