Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa

Kinya Toriyama, Kazunori Hanaoka, Takashi Okada, Masao Watanabe

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

A polyclonal antiserum was raised against the extracellular pollen proteins of Brassica rapa and used for screening the expression cDNA libraries made from immature anthers. We obtained five groups of cDNA clones, including cDNAs similar to PCP1, thioredoxin, and lipid transfer protein (LTP). Recombinant protein of the cDNA clone showing sequence similarity to LTP was demonstrated to bind IgE of a patient allergic to Brassica pollen. The cDNA clone reported here, therefore, represents a novel pollen allergen of Brassica rapa.

Original languageEnglish
Pages (from-to)234-238
Number of pages5
JournalFEBS Letters
Volume424
Issue number3
DOIs
Publication statusPublished - 1998 Mar 13

Keywords

  • Anther
  • Brassica rapa
  • Immunoglobulin E
  • Lipid transfer protein
  • Pollen allergen
  • cDNA cloning

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa'. Together they form a unique fingerprint.

Cite this