Molecular cloning of a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A

Osamu Tanabe, Terumasa Nagase, Takehiko Murakami, Hideto Nozaki, Hirofumi Usui, Yasumasa Nishito, Hideyuki Hayashi, Hiroyuki Kagamiyama, Masao Takeda

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49 Citations (Scopus)

Abstract

Based on amino acid sequence data of a 74-kDa regulatory subunit (B″ or δ) of a human heterotrimeric protein phosphatase 2A, a cDNA encoding the subunit was isolated from a human cerebral cortex library. The cDNA had an open reading frame encoding an Mr 66 138 protein of 570 amino acids. Bacterial expression of the cDNA yielded a protein immunoreactive with antisera specific to the 74-kDa subunit. The predicted primary structure of the subunit had no similarity to already reported sequences of PP2A regulatory subunits including A, B, and PR72. Potential phosphorylation sites for protein kinases A and C, a bipartite motif of putative nuclear localization signal, an SH3 accessible proline-rich domain, and a unique PQ repeat were found in the sequence. The subunit mRN A of about 2.9 kb was ubiquitously expressed in rat tissues.

Original languageEnglish
Pages (from-to)107-111
Number of pages5
JournalFEBS Letters
Volume379
Issue number1
DOIs
Publication statusPublished - 1996 Jan 22

Keywords

  • 74-kDa regulatory subunit
  • Cloning
  • Human cerebral cortex
  • Human erythrocyte
  • Protein phosphatase 2A

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Tanabe, O., Nagase, T., Murakami, T., Nozaki, H., Usui, H., Nishito, Y., Hayashi, H., Kagamiyama, H., & Takeda, M. (1996). Molecular cloning of a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A. FEBS Letters, 379(1), 107-111. https://doi.org/10.1016/0014-5793(95)01500-0