TY - JOUR
T1 - Molecular cloning of a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A
AU - Tanabe, Osamu
AU - Nagase, Terumasa
AU - Murakami, Takehiko
AU - Nozaki, Hideto
AU - Usui, Hirofumi
AU - Nishito, Yasumasa
AU - Hayashi, Hideyuki
AU - Kagamiyama, Hiroyuki
AU - Takeda, Masao
N1 - Funding Information:
Acknowledgements: We are grateful to Dr. Jean Lucus-Lenard (University of Connecticut) for her critical reading of the manuscript. We thank Dr. Shigetada Nakanishi (Kyoto University) for providing a human cerebral cortex cDNA library, and Dr. Takuya Shimamoto (Osaka University) for providing HeLa cell cDNA. We also thank Mieko Kawamura for her excellent secretarial assistance and Ryoko Takemoto for her skillful technical assistance. This work was supported in part by Grants-in-Aid for Cancer Research and Scientific Research from the Ministry of Education, Science, and Culture of Japan (1990-1995), and a grant from the Kanae Foundation of Research for New Medicine, Osaka, Japan (1994).
PY - 1996/1/22
Y1 - 1996/1/22
N2 - Based on amino acid sequence data of a 74-kDa regulatory subunit (B″ or δ) of a human heterotrimeric protein phosphatase 2A, a cDNA encoding the subunit was isolated from a human cerebral cortex library. The cDNA had an open reading frame encoding an Mr 66 138 protein of 570 amino acids. Bacterial expression of the cDNA yielded a protein immunoreactive with antisera specific to the 74-kDa subunit. The predicted primary structure of the subunit had no similarity to already reported sequences of PP2A regulatory subunits including A, B, and PR72. Potential phosphorylation sites for protein kinases A and C, a bipartite motif of putative nuclear localization signal, an SH3 accessible proline-rich domain, and a unique PQ repeat were found in the sequence. The subunit mRN A of about 2.9 kb was ubiquitously expressed in rat tissues.
AB - Based on amino acid sequence data of a 74-kDa regulatory subunit (B″ or δ) of a human heterotrimeric protein phosphatase 2A, a cDNA encoding the subunit was isolated from a human cerebral cortex library. The cDNA had an open reading frame encoding an Mr 66 138 protein of 570 amino acids. Bacterial expression of the cDNA yielded a protein immunoreactive with antisera specific to the 74-kDa subunit. The predicted primary structure of the subunit had no similarity to already reported sequences of PP2A regulatory subunits including A, B, and PR72. Potential phosphorylation sites for protein kinases A and C, a bipartite motif of putative nuclear localization signal, an SH3 accessible proline-rich domain, and a unique PQ repeat were found in the sequence. The subunit mRN A of about 2.9 kb was ubiquitously expressed in rat tissues.
KW - 74-kDa regulatory subunit
KW - Cloning
KW - Human cerebral cortex
KW - Human erythrocyte
KW - Protein phosphatase 2A
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U2 - 10.1016/0014-5793(95)01500-0
DO - 10.1016/0014-5793(95)01500-0
M3 - Article
C2 - 8566219
AN - SCOPUS:0030035065
VL - 379
SP - 107
EP - 111
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -