Molecular cloning of a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A

Osamu Tanabe; Terumasa Nagase; Takehiko Murakami; Hideto Nozaki; Hirofumi Usui; Yasumasa Nishito; Hideyuki Hayashi; Hiroyuki Kagamiyama; Masao Takeda

doi:10.1016/0014-5793(95)01500-0

Molecular cloning of a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A

Osamu Tanabe, Terumasa Nagase, Takehiko Murakami, Hideto Nozaki, Hirofumi Usui, Yasumasa Nishito, Hideyuki Hayashi, Hiroyuki Kagamiyama, Masao Takeda

Tohoku Medical Megabank Organization (ToMMo)

Research output: Contribution to journal › Article

49 Citations (Scopus)

Abstract

Based on amino acid sequence data of a 74-kDa regulatory subunit (B″ or δ) of a human heterotrimeric protein phosphatase 2A, a cDNA encoding the subunit was isolated from a human cerebral cortex library. The cDNA had an open reading frame encoding an M_r 66 138 protein of 570 amino acids. Bacterial expression of the cDNA yielded a protein immunoreactive with antisera specific to the 74-kDa subunit. The predicted primary structure of the subunit had no similarity to already reported sequences of PP2A regulatory subunits including A, B, and PR72. Potential phosphorylation sites for protein kinases A and C, a bipartite motif of putative nuclear localization signal, an SH3 accessible proline-rich domain, and a unique PQ repeat were found in the sequence. The subunit mRN A of about 2.9 kb was ubiquitously expressed in rat tissues.

Original language	English
Pages (from-to)	107-111
Number of pages	5
Journal	FEBS Letters
Volume	379
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(95)01500-0
Publication status	Published - 1996 Jan 22

Keywords

74-kDa regulatory subunit
Cloning
Human cerebral cortex
Human erythrocyte
Protein phosphatase 2A

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Tanabe, O., Nagase, T., Murakami, T., Nozaki, H., Usui, H., Nishito, Y., Hayashi, H., Kagamiyama, H., & Takeda, M. (1996). Molecular cloning of a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A. FEBS Letters, 379(1), 107-111. https://doi.org/10.1016/0014-5793(95)01500-0

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abstract = "Based on amino acid sequence data of a 74-kDa regulatory subunit (B″ or δ) of a human heterotrimeric protein phosphatase 2A, a cDNA encoding the subunit was isolated from a human cerebral cortex library. The cDNA had an open reading frame encoding an Mr 66 138 protein of 570 amino acids. Bacterial expression of the cDNA yielded a protein immunoreactive with antisera specific to the 74-kDa subunit. The predicted primary structure of the subunit had no similarity to already reported sequences of PP2A regulatory subunits including A, B, and PR72. Potential phosphorylation sites for protein kinases A and C, a bipartite motif of putative nuclear localization signal, an SH3 accessible proline-rich domain, and a unique PQ repeat were found in the sequence. The subunit mRN A of about 2.9 kb was ubiquitously expressed in rat tissues.",

keywords = "74-kDa regulatory subunit, Cloning, Human cerebral cortex, Human erythrocyte, Protein phosphatase 2A",

author = "Osamu Tanabe and Terumasa Nagase and Takehiko Murakami and Hideto Nozaki and Hirofumi Usui and Yasumasa Nishito and Hideyuki Hayashi and Hiroyuki Kagamiyama and Masao Takeda",

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AU - Tanabe, Osamu

AU - Nagase, Terumasa

AU - Murakami, Takehiko

AU - Nozaki, Hideto

AU - Usui, Hirofumi

AU - Nishito, Yasumasa

AU - Hayashi, Hideyuki

AU - Kagamiyama, Hiroyuki

AU - Takeda, Masao

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N2 - Based on amino acid sequence data of a 74-kDa regulatory subunit (B″ or δ) of a human heterotrimeric protein phosphatase 2A, a cDNA encoding the subunit was isolated from a human cerebral cortex library. The cDNA had an open reading frame encoding an Mr 66 138 protein of 570 amino acids. Bacterial expression of the cDNA yielded a protein immunoreactive with antisera specific to the 74-kDa subunit. The predicted primary structure of the subunit had no similarity to already reported sequences of PP2A regulatory subunits including A, B, and PR72. Potential phosphorylation sites for protein kinases A and C, a bipartite motif of putative nuclear localization signal, an SH3 accessible proline-rich domain, and a unique PQ repeat were found in the sequence. The subunit mRN A of about 2.9 kb was ubiquitously expressed in rat tissues.

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KW - 74-kDa regulatory subunit

KW - Cloning

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KW - Human erythrocyte

KW - Protein phosphatase 2A

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