Molecular cloning, characterization and expression of an elongation factor 1α gene in maize

Thomas Berberich, Kazuyuki Sugawara, Mariko Harada, Tomonobu Kusano

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    57 Citations (Scopus)

    Abstract

    A cDNA (zmEF1A) and the corresponding genomic clone (zmgEF1A) of a member of the gene family encoding the α subunit of translation elongation factor 1 (EF-1α) have been isolated from maize. The deduced amino acid sequence is 447 residues long interrupted by one intron. Southern blot analysis reveals that the cloned EF-1α gene is one member out of a family consisting of at least six genes. As shown by northern hybridizations in leaves the mRNA level increases at low temperature whereas time-course experiments over 24 h at 5°C show that in roots the overall mRNA level of EF-1α is transiently decreased. These results indicate that the expression of EF-1α is differently regulated in leaves and roots under cold stress.

    Original languageEnglish
    Pages (from-to)611-615
    Number of pages5
    JournalPlant Molecular Biology
    Volume29
    Issue number3
    DOIs
    Publication statusPublished - 1995 Nov 1

    Keywords

    • EF-1α
    • Zea mays
    • cDNA sequence
    • elongation factor 1α
    • gene

    ASJC Scopus subject areas

    • Biochemistry
    • Biochemistry, Genetics and Molecular Biology(all)

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