Molecular cloning and sequence analysis of cDNA for the catalytic subunit 1α of rat kidney type 1 protein phosphatase, and detection of the gene expression at high levels in hepatoma cells and regenerating livers as compared to rat livers

Kazuki Kitamura, Yusuke Mizuno, Akio Sasaki, Akira Yasui, Shigeru Tsuiki, Kunimi Kikuchi

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    26 Citations (Scopus)

    Abstract

    A cDNA clone containing the full coding sequence of a type 1 protein phosphatase catalytic subunit lα has been isolated from a rat kidney λgt 10 library. The protein sequence deduced from the cDNA contains 330 ami no acid residues with a molecular mass of 38 kDa. The cDNA clone from rat kidney was 89% identical at the nucleotide level in the coding region to type 1 protein phosphatase lα from rabbit skeletal muscle. However, the two protein sequences were completely identical. The type lα protein phosphatase from rat kidney shows 49% homology of amino acid sequence to the rat type 2Aα protein phosphatase. Thus, the protein sequence of type 1α protein phosphatase was completely conserved between rat and rabbit. The mRNA levels of type 1 protein phosphatase were determined in rat liver, AH13, a strain of rat hepatoma, and regenerating rat liver by Northern blot analysis using the cDNA fragment as a probe, under which conditions a single mRNA of 1.5 kb was detected. The mRNA levels of AH13 were remarkably increased when compared to those of normal livers, whereas the mRNA levels of regenerating livers were slightly but significantly increased. These results demonstrate a marked increase in gene expression of type 1 protein phosphatase in hepatoma cells, suggesting an important role of the type 1 protein phosphatase in hepatocarcinogenesis.

    Original languageEnglish
    Pages (from-to)307-310
    Number of pages4
    JournalJournal of biochemistry
    Volume109
    Issue number2
    Publication statusPublished - 1991 Feb 1

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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