Molecular cloning and partial characterization of a tobacco cDNA encoding a small bZIP protein

Tomonobu Kusano; Kazuyuki Sugawara; Mariko Harada; Thomas Berberich

doi:10.1016/S0167-4781(97)00161-9

Molecular cloning and partial characterization of a tobacco cDNA encoding a small bZIP protein

Tomonobu Kusano, Kazuyuki Sugawara, Mariko Harada, Thomas Berberich

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Here we report the isolation of a tobacco cDNA which is a counterpart gene of rice low-temperature-induced lip19. Expression of the gene (termed tbz17) is also positively controlled by low temperature but fairly moderately compared with the low-temperature-responsiveness of rice lip19 and the maize counterpart gene, mlip15. The predicted gene product (termed TBZ17) is a bZIP protein of 145 amino acids and shows about 45% identity with rice LIP19 and maize mLIP15 proteins. DNA binding studies indicate that TBZI7 has quite similar DNA binding characteristics to that of mLIP15. The results suggest that low-temperature-induced gene(s) encoding a DNA binding factor with bZIP motif is omni-present in higher plants.

Original language	English
Pages (from-to)	171-175
Number of pages	5
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1395
Issue number	2
DOIs	https://doi.org/10.1016/S0167-4781(97)00161-9
Publication status	Published - 1998 Jan 21

Keywords

DNA binding
Low temperature
Nicotiana tabacum
bZIP protein

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(97)00161-9

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title = "Molecular cloning and partial characterization of a tobacco cDNA encoding a small bZIP protein",

abstract = "Here we report the isolation of a tobacco cDNA which is a counterpart gene of rice low-temperature-induced lip19. Expression of the gene (termed tbz17) is also positively controlled by low temperature but fairly moderately compared with the low-temperature-responsiveness of rice lip19 and the maize counterpart gene, mlip15. The predicted gene product (termed TBZ17) is a bZIP protein of 145 amino acids and shows about 45% identity with rice LIP19 and maize mLIP15 proteins. DNA binding studies indicate that TBZI7 has quite similar DNA binding characteristics to that of mLIP15. The results suggest that low-temperature-induced gene(s) encoding a DNA binding factor with bZIP motif is omni-present in higher plants.",

keywords = "DNA binding, Low temperature, Nicotiana tabacum, bZIP protein",

author = "Tomonobu Kusano and Kazuyuki Sugawara and Mariko Harada and Thomas Berberich",

note = "Funding Information: We thank Drs. V. Hemleben and M. Iwabuchi for providing plasmids. T.B. acknowledges the financial support from the E. C. Scientific Exchange Programme in Japan. This research was supported by a grant from the Ministry of Agriculture, Forestry and Fishery and by Grant RFTF 96L00602 from the Japan Society for Promotion of Science to T.K. ",

year = "1998",

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doi = "10.1016/S0167-4781(97)00161-9",

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T1 - Molecular cloning and partial characterization of a tobacco cDNA encoding a small bZIP protein

AU - Kusano, Tomonobu

AU - Sugawara, Kazuyuki

AU - Harada, Mariko

AU - Berberich, Thomas

N1 - Funding Information: We thank Drs. V. Hemleben and M. Iwabuchi for providing plasmids. T.B. acknowledges the financial support from the E. C. Scientific Exchange Programme in Japan. This research was supported by a grant from the Ministry of Agriculture, Forestry and Fishery and by Grant RFTF 96L00602 from the Japan Society for Promotion of Science to T.K.

PY - 1998/1/21

Y1 - 1998/1/21

N2 - Here we report the isolation of a tobacco cDNA which is a counterpart gene of rice low-temperature-induced lip19. Expression of the gene (termed tbz17) is also positively controlled by low temperature but fairly moderately compared with the low-temperature-responsiveness of rice lip19 and the maize counterpart gene, mlip15. The predicted gene product (termed TBZ17) is a bZIP protein of 145 amino acids and shows about 45% identity with rice LIP19 and maize mLIP15 proteins. DNA binding studies indicate that TBZI7 has quite similar DNA binding characteristics to that of mLIP15. The results suggest that low-temperature-induced gene(s) encoding a DNA binding factor with bZIP motif is omni-present in higher plants.

AB - Here we report the isolation of a tobacco cDNA which is a counterpart gene of rice low-temperature-induced lip19. Expression of the gene (termed tbz17) is also positively controlled by low temperature but fairly moderately compared with the low-temperature-responsiveness of rice lip19 and the maize counterpart gene, mlip15. The predicted gene product (termed TBZ17) is a bZIP protein of 145 amino acids and shows about 45% identity with rice LIP19 and maize mLIP15 proteins. DNA binding studies indicate that TBZI7 has quite similar DNA binding characteristics to that of mLIP15. The results suggest that low-temperature-induced gene(s) encoding a DNA binding factor with bZIP motif is omni-present in higher plants.

KW - DNA binding

KW - Low temperature

KW - Nicotiana tabacum

KW - bZIP protein

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

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Molecular cloning and partial characterization of a tobacco cDNA encoding a small bZIP protein

Abstract

Keywords

ASJC Scopus subject areas

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