Molecular cloning and nucleotide sequence of human pancreatic prechymotrypsinogen cDNA

Naohiro Tomita, Yoshitaka Izumoto, Akira Horii, Sadayuki Doi, Hideoki Yokouchi, Michio Ogawa, Takesada Mori, Kenichi Matsubara

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

The cDNA clone encoding human prechymotrypsinogen was isolated from a human pancreas cDNA library and its nucleotide sequence was determined. The sequence consists of a 16 bp 5′ non-coding region, a 789 bp amino acid coding region and a 60 bp 3′ non-coding region. The predicted product consists of 263 amino acids, including 18 amino acids for a signal peptide and 15 amino acids possible for an activation peptide. Southern blot analyses using the cloned cDNA as a probe revealed that human genomic DNA carries at least two genes that are related to chymotrypsinogen.

Original languageEnglish
Pages (from-to)569-575
Number of pages7
JournalBiochemical and biophysical research communications
Volume158
Issue number2
DOIs
Publication statusPublished - 1989 Jan 31

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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