The cDNA clone encoding human prechymotrypsinogen was isolated from a human pancreas cDNA library and its nucleotide sequence was determined. The sequence consists of a 16 bp 5′ non-coding region, a 789 bp amino acid coding region and a 60 bp 3′ non-coding region. The predicted product consists of 263 amino acids, including 18 amino acids for a signal peptide and 15 amino acids possible for an activation peptide. Southern blot analyses using the cloned cDNA as a probe revealed that human genomic DNA carries at least two genes that are related to chymotrypsinogen.
|Number of pages||7|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - 1989 Jan 31|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology