Molecular Cloning and Intrarenal Localization of Rat Prostaglandin E2 Receptor EP3 Subtype

Kazuhisa Takeuchi, Takaaki Abe, Nobuyuki Takahashi, Keishi Abe

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

A rat kidney cDNA library was screened with a mouse prostaglandin (PG) E2 receptor EP subtype cDNA as a probe, and a 2.2-kilobase pair cDNA was isolated. The cDNA encodes 365 amino acids with 97.0% sequence identity to mouse EP receptor. Specific binding of [3H]PGE2 was found in COS-7 cells transfected with the cDNA (Kd=3.2nM) and was displaced with unlabeled prostaglandins in the order of PGE2 = PGE1 >> PGF > PGD2. Thus, a cDNA for rat EP3 (rEP3) receptor was cloned. In situ hybridization revealed expression of rEP3 receptor principally in tubules of renal medulla. Consistent with this observation, the reverse transcription and polymerase chain reaction (RT-PCR) technique using dissected nephron segments showed the receptor expression specifically in medullary thick ascending limbs of Henle’s loop (mTAL), cortical TAL (cTAL), cortical collecting ducts (CCD) and inner medullary collecting ducts (IMCD), indicating a possible diuretic and natriuretic role of rEP3 receptor at the distal nephron segments.

Original languageEnglish
Pages (from-to)885-891
Number of pages7
JournalBiochemical and biophysical research communications
Volume194
Issue number2
DOIs
Publication statusPublished - 1993 Jul 30

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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