Molecular cloning and expression of retinoic-acid synthesizing enzyme raldh2 from Takifugu rubripes

Susumu Uji, Tohru Suzuki, Tadahide Kurokawa

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Retinaldehyde dehydrogenases (raldhs) synthesize retinoic acid (RA), which is required for pattern formation and organogenesis during embryogenesis. To elucidate the common role of RA on vertebrate embryos, we first sought to clone a homologous gene to human raldh2 from fugu, Takifugu rubripes. We cloned a 1837 bp cDNA that encodes fugu raldh. The deduced amino acid sequence of the fugu raldh comprises 502 amino acids. The fugu Raldh showed highest sequence identity to zebrafish, Danio rerio, Raldh2 (79.9%). The fugu Raldh also showed high sequence identity to other vertebrate Raldh2: Xenopus laevis (77.2%), human (77.4%), mouse (74.3%) and chick (73.9%). Comparative genomic analysis showed that the gene arrangement around fugu raldh agreed with that of human raldh2. Fugu raldh mRNA was expressed through embryogenesis similarly to raldh2 in other vertebrates. These results and phylogenetic analyses suggest that pufferfish raldh is a fugu orthologue of other species' raldh2.

Original languageEnglish
Pages (from-to)133-138
Number of pages6
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume1
Issue number1 SPEC. ISS.
DOIs
Publication statusPublished - 2006 Mar

Keywords

  • Embryogenesis
  • Genome
  • RT-PCR
  • Raldh
  • Retinoic acid
  • Takifugu rubripes
  • Vitamin A
  • cDNA

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Genetics

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