Molecular cloning and expression of gelatinases (MMP-2 and MMP-9) in the pufferfish Takifugu rubripes

Hiroshi Tsukamoto, Yoshihiro Yokoyama, Tohru Suzuki, Shoshi Mizuta, Reiji Yoshinaka

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

To determine the metabolism location of the extra-cellular matrix proteins in fugu (Takifugu rubripes), we cloned the cDNAs of the fugu gelatinases, matrix metalloproteinase-2 (MMP-2) and MMP-9, and examined their expressions in various adult tissues using a quantitative real-time PCR. The expression profiles of fugu gelatinases were different among tissues. FgMMP-9 mRNA was abundant in tissues that contain blood cells abundantly where fgMMP-2 mRNA was little expressed. We also examined the expression of these genes in fugu embryos during development using a whole mount in situ hybridization. Fugu MMP-2 mRNA was expressed in the pharyngeal area and mesenchyme in embryos at 80 hours post fertilization (hpf). While fugu MMP-9 mRNA was expressed in the vent at 140 hpf and the caudal end of the fin fold at 172 hpf. Although fugu MMP-2 mRNA was expressed in the pectoral fin bud at 120 hpf, fugu MMP-9 mRNA did not appear in this tissue until 10 days post fertilization (dpf). These data show expression profiles differ between the fugu gelatinases and suggest expressions of these genes are controlled at the matrix protein degradation site in fugu embryos during development.

Original languageEnglish
Pages (from-to)295-302
Number of pages8
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume148
Issue number3
DOIs
Publication statusPublished - 2007 Nov

Keywords

  • Embryo
  • Fugu
  • Gelatinases
  • MMP-9
  • Matrix metalloproteinase (MMP)-2
  • Quantitative real-time PCR
  • Takifugu rubripes
  • Teleost
  • Whole mount in situ hybridization

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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