Molecular cloning and expression of a rat cDNA encoding MDCK-type chloride channel

Takaaki Abe, Kazuhisa Takeuchi, Kuniaki Ishii, Keishi Abe

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

A cDNA encoding MDCK-type chloride channel was cloned from the rat kidney cDNA library using the homology screening approach. The deduced amino acid sequence of cloned cDNA has a 92.4% homology with the MDCK chloride channel, and electrophysiological analysis using Xenopus oocyte expression system showed the functional character of chloride channel. The rat kidney chloride channel, abbreviated RKCL, is not specific to the kidney, but widely expressed in various tissues. It is suggested that RKCL is important for most cells.

Original languageEnglish
Pages (from-to)353-356
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1173
Issue number3
DOIs
Publication statusPublished - 1993 Jun 25

Keywords

  • (Rat)
  • (Xenopus)
  • A7r5
  • Chloride channel
  • Gene expression
  • Oocyte expression

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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