Molecular cloning and characterization of coffee cDNA encoding spermidine synthase

Tomoko Hatanaka, Hiroshi Sano, Tomonobu Kusano

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    A cDNA for spermidine synthase (SPDS), which converts putrescine to spermidine using decarboxylated S-adenosylmethionine as a co-factor, has been isolated from Coffea arabica. When the SPDS cDNA is expressed in an SPDS- deficient E. coli mutant, the recombinant protein shows high SPDS activity. The C. arabica SPDS possesses the co-factor binding motifs which have been proposed for S-adenosylmethionine, and its amino acid sequence is similar to other plant SPDSs. The SPDS transcripts have been observed in roots, green stems, old and young leaves, and accumulated to a higher level in rapid growing tissues, such as green stems and younger leaves, compared to old leaves. In callus tissues, it has been expressed in all stages, even though callus growth is very slow during somatic embryogenesis.

    Original languageEnglish
    Pages (from-to)161-168
    Number of pages8
    JournalPlant Science
    Volume140
    Issue number2
    DOIs
    Publication statusPublished - 1999 Jan 25

    Keywords

    • Callus culture
    • Coffea arabica
    • Gene cloning
    • Polyamine
    • Somatic embryogenesis
    • Spermidine synthase (EC 2.5.1.16)

    ASJC Scopus subject areas

    • Genetics
    • Agronomy and Crop Science
    • Plant Science

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