Molecular cloning and characterization of a human brain ryanodine receptor

Yasuyo Nakashima, Seiichiro Nishimura, Akito Maeda, Edward L. Barsoumian, Yasuhiro Hakamata, Junichi Nakai, Paul D. Allen, Keiji Imoto, Toru Kita

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We have cloned and sequenced the cDNA of the human brain ryanodine receptor (RyR3), which is composed of 4866 nmino acids and shares characteristic structural features with the rabbit RyR3. Northern blot analysis shows that the human RyR3 mRNA is abundantly expressed in hippocampus, caudate nucleus and amygdala as well as in skeletal muscle. The human RyR3 mRNA is also detected in several cell lines derived from human brain tumors. Functional expression of RyR3 and a chimeric RyR suggests that RyR3 forms a calcium-release channel with a very low Ca2+ sensitivity.

Original languageEnglish
Pages (from-to)157-162
Number of pages6
JournalFEBS Letters
Volume417
Issue number1
DOIs
Publication statusPublished - 1997 Nov 3
Externally publishedYes

Keywords

  • Calcium release channel
  • Chimeric RyR
  • Functional expression
  • RNA blot hybridization
  • Ryanodine receptor
  • cDNA cloning

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Nakashima, Y., Nishimura, S., Maeda, A., Barsoumian, E. L., Hakamata, Y., Nakai, J., Allen, P. D., Imoto, K., & Kita, T. (1997). Molecular cloning and characterization of a human brain ryanodine receptor. FEBS Letters, 417(1), 157-162. https://doi.org/10.1016/S0014-5793(97)01275-1