Molecular cloning and characterization of a human brain ryanodine receptor

Yasuyo Nakashima; Seiichiro Nishimura; Akito Maeda; Edward L. Barsoumian; Yasuhiro Hakamata; Junichi Nakai; Paul D. Allen; Keiji Imoto; Toru Kita

doi:10.1016/S0014-5793(97)01275-1

Molecular cloning and characterization of a human brain ryanodine receptor

Yasuyo Nakashima, Seiichiro Nishimura, Akito Maeda, Edward L. Barsoumian, Yasuhiro Hakamata, Junichi Nakai, Paul D. Allen, Keiji Imoto, Toru Kita

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

We have cloned and sequenced the cDNA of the human brain ryanodine receptor (RyR3), which is composed of 4866 nmino acids and shares characteristic structural features with the rabbit RyR3. Northern blot analysis shows that the human RyR3 mRNA is abundantly expressed in hippocampus, caudate nucleus and amygdala as well as in skeletal muscle. The human RyR3 mRNA is also detected in several cell lines derived from human brain tumors. Functional expression of RyR3 and a chimeric RyR suggests that RyR3 forms a calcium-release channel with a very low Ca²⁺ sensitivity.

Original language	English
Pages (from-to)	157-162
Number of pages	6
Journal	FEBS Letters
Volume	417
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(97)01275-1
Publication status	Published - 1997 Nov 3
Externally published	Yes

Keywords

Calcium release channel
Chimeric RyR
Functional expression
RNA blot hybridization
Ryanodine receptor
cDNA cloning

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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abstract = "We have cloned and sequenced the cDNA of the human brain ryanodine receptor (RyR3), which is composed of 4866 nmino acids and shares characteristic structural features with the rabbit RyR3. Northern blot analysis shows that the human RyR3 mRNA is abundantly expressed in hippocampus, caudate nucleus and amygdala as well as in skeletal muscle. The human RyR3 mRNA is also detected in several cell lines derived from human brain tumors. Functional expression of RyR3 and a chimeric RyR suggests that RyR3 forms a calcium-release channel with a very low Ca2+ sensitivity.",

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AU - Nakashima, Yasuyo

AU - Nishimura, Seiichiro

AU - Maeda, Akito

AU - Barsoumian, Edward L.

AU - Hakamata, Yasuhiro

AU - Nakai, Junichi

AU - Allen, Paul D.

AU - Imoto, Keiji

AU - Kita, Toru

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KW - Chimeric RyR

KW - Functional expression

KW - RNA blot hybridization

KW - Ryanodine receptor

KW - cDNA cloning

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Molecular cloning and characterization of a human brain ryanodine receptor

Abstract

Keywords

ASJC Scopus subject areas

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