TY - JOUR
T1 - Molecular cloning and characterization of a human brain ryanodine receptor
AU - Nakashima, Yasuyo
AU - Nishimura, Seiichiro
AU - Maeda, Akito
AU - Barsoumian, Edward L.
AU - Hakamata, Yasuhiro
AU - Nakai, Junichi
AU - Allen, Paul D.
AU - Imoto, Keiji
AU - Kita, Toru
N1 - Funding Information:
We thank Ms. Miwa Kataoka for technical assistance. This investigation was supported in part by research grants from the Ministry of Education, Science and Culture of Japan and Shimizu Immunological Research Grant for 1995 and NIH R01AR43301 and MDA (P.D.A.).
PY - 1997/11/3
Y1 - 1997/11/3
N2 - We have cloned and sequenced the cDNA of the human brain ryanodine receptor (RyR3), which is composed of 4866 nmino acids and shares characteristic structural features with the rabbit RyR3. Northern blot analysis shows that the human RyR3 mRNA is abundantly expressed in hippocampus, caudate nucleus and amygdala as well as in skeletal muscle. The human RyR3 mRNA is also detected in several cell lines derived from human brain tumors. Functional expression of RyR3 and a chimeric RyR suggests that RyR3 forms a calcium-release channel with a very low Ca2+ sensitivity.
AB - We have cloned and sequenced the cDNA of the human brain ryanodine receptor (RyR3), which is composed of 4866 nmino acids and shares characteristic structural features with the rabbit RyR3. Northern blot analysis shows that the human RyR3 mRNA is abundantly expressed in hippocampus, caudate nucleus and amygdala as well as in skeletal muscle. The human RyR3 mRNA is also detected in several cell lines derived from human brain tumors. Functional expression of RyR3 and a chimeric RyR suggests that RyR3 forms a calcium-release channel with a very low Ca2+ sensitivity.
KW - Calcium release channel
KW - Chimeric RyR
KW - Functional expression
KW - RNA blot hybridization
KW - Ryanodine receptor
KW - cDNA cloning
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U2 - 10.1016/S0014-5793(97)01275-1
DO - 10.1016/S0014-5793(97)01275-1
M3 - Article
C2 - 9395096
AN - SCOPUS:0030671538
VL - 417
SP - 157
EP - 162
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -