Molecular cloning and characterization of a cDNA for a novel ethylene receptor, NT-ERS1, of tobacco (Nicotiana tabacum L.)

Yoshifumi Terajima, Hideki Nukui, Akie Kobayashi, Shin Fujimoto, Shuu Hase, Toshihito Yoshioka, Teruyoshi Hashiba, Shigeru Satoh

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

The cDNA encoding a novel member (NT-ERS1) of ethylene receptor family of tobacco (Nicotiana tabacum L.) was obtained by a combination of RT-PCR and 5′-/3′-RACE cloning. The cDNA was 2,092 nucleotides long and had an open reading frame of 1,911 bp encoding 637 amino acids. The deduced polypeptide lacked a response regulator domain, indicating that the ethylene receptor belongs to an ERS-group. The amino acid sequence was similar to respective members of the tobacco ethylene receptor family: 67.8% to NT-ETR1, 39.1% to NTHK1 and 31.1% to NTHK2. Comparison of amino acid sequence suggested that NT-ERS1 is the counterpart of Nr in the ethylene receptor family of tomato, which belongs to Solanaceae as does tobacco. Northern blot analysis showed that mRNA of NT-ERS1 was present in leaf, shoot and root tissues, and accumulated in leaves treated with exogenous ethylene. A mutated NT-ERS1 cDNA transgene, obtained by introducing one nucleotide substitution into NT-ETR1 cDNA, conferred ethylene insensitivity in tobacco plants, indicating that the translation product of the cDNA actually functioned in the plants.

Original languageEnglish
Pages (from-to)308-313
Number of pages6
JournalPlant and Cell Physiology
Volume42
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • Ethylene receptor genes
  • Ethylene-insensitive transgenic tobacco
  • Nicotiana tabacum
  • Solanaceae
  • Tobacco

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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