Molecular cloning and cDNA sequence analysis of carboxypeptidases A1, A2 and B from the Japanese flounder Paralichthys olivaceus

Anand S. Srivastava, Tadahide Kurokawa, Tohru Suzuki

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Although pancreatic serine proteases have been cloned in teleosts, no sequence data are currently available on members of the carboxypeptidase (CP) family. Here, we cloned cDNAs coding for two preproCPAs, corresponding to mammalian preproCPA1 and preproCPA2, and one preproCPB from a pancreatic cDNA library of the Japanese flounder, Paralichthys olivaceus. The activation peptides of flounder proCPs completely retained the sequences for inhibition of enzymatic activity of proCPs just like mammalian proCPs. Of 306-309 amino acids in total, 95 amino acids are completely conserved between bovine CPA1 and CPB and flounder CPs. Notably, amino acid residues for Zn2+ ligands, catalysis and substrate anchoring are completely conserved between flounder and bovine CPs. Three species of flounder preproCPs are all expressed in the pancreas of first feeding larvae.

Original languageEnglish
Pages (from-to)593-599
Number of pages7
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume135
Issue number4
DOIs
Publication statusPublished - 2003 Aug 1
Externally publishedYes

Keywords

  • Carboxypeptidase A1
  • Carboxypeptidase A2
  • Carboxypeptidase B
  • Flounder
  • Pancreas
  • cDNA Teleost

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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