Molecular characterization of the human excision repair gene ERCC-1: cDNA cloning and amino acid homology with the yeast DNA repair gene RAD10

Marcel van Duin, Jan de Wit, Hanny Odijk, Andries Westerveld, Akira Yasui, Marcel H.M. Koken, Jan H.J. Hoeijmakers, Dirk Bootsma

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    258 Citations (Scopus)

    Abstract

    The human excision repair gene ERCC-1 was cloned after DNA mediated gene transfer to the CHO mutant 43-3B, which is sensitive to ultraviolet light and mitomycin-C. We describe the cloning and sequence analysis of the ERCC-1 cDNA and partial characterization of the gene. ERCC-1 has a size of 15 kb and is located on human chromosome 19. The ERCC-1 precursor RNA is subject to alternative splicing of an internal 72 bp coding exon. Only the cDNA of the larger 1.1 kb transcript, encoding a protein of 297 amino acids, was able to confer resistance to ultraviolet light and mitomycin-C on 43-3B cells. Significant amino acid sequence homology was found between the ERCC-1 gene product and the yeast excision repair protein RAD10. The most homologous region displayed structural homology with DNA binding domains of various polypeptides.

    Original languageEnglish
    Pages (from-to)913-923
    Number of pages11
    JournalCell
    Volume44
    Issue number6
    DOIs
    Publication statusPublished - 1986 Mar 28

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

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    van Duin, M., de Wit, J., Odijk, H., Westerveld, A., Yasui, A., Koken, M. H. M., Hoeijmakers, J. H. J., & Bootsma, D. (1986). Molecular characterization of the human excision repair gene ERCC-1: cDNA cloning and amino acid homology with the yeast DNA repair gene RAD10. Cell, 44(6), 913-923. https://doi.org/10.1016/0092-8674(86)90014-0