Molecular characterization of rice genes specifically expressed in the anther tapetum

Tohru Tsuchiya; Kinya Toriyama; Shin ichiro Ejiri; Kokichi Hinata

doi:10.1007/BF00019488

Molecular characterization of rice genes specifically expressed in the anther tapetum

Tohru Tsuchiya, Kinya Toriyama, Shin ichiro Ejiri, Kokichi Hinata

AGR - Life Sciences

Research output: Contribution to journal › Article › peer-review

81 Citations (Scopus)

Abstract

In situ localization of mRNA was carried out on two cDNAs (Osc4 and Osc6) that had been isolated from rice anthers at the microspore stage. The mRNA corresponding to each cDNA was shown to be localized only in the tapetal cells of the rice immature anthers, but not in the microspores or the mature pollen. The corresponding genomic clone, Osg6B, was isolated, and its 5′-upstream region was found to regulate β-glucuronidase expression in the tapetum of transgenic tobacco. A set of 5′ deletions was also generated and a 1095 bp 5′ region was revealed to be necessary for activation of the Osg6B promoter in transgenic tobacco.

Original language	English
Pages (from-to)	1737-1746
Number of pages	10
Journal	Plant Molecular Biology
Volume	26
Issue number	6
DOIs	https://doi.org/10.1007/BF00019488
Publication status	Published - 1994 Dec 1

Keywords

anther
in situ hybridization
promoter analysis
rice
tapetum
transgenic tobacco

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

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title = "Molecular characterization of rice genes specifically expressed in the anther tapetum",

abstract = "In situ localization of mRNA was carried out on two cDNAs (Osc4 and Osc6) that had been isolated from rice anthers at the microspore stage. The mRNA corresponding to each cDNA was shown to be localized only in the tapetal cells of the rice immature anthers, but not in the microspores or the mature pollen. The corresponding genomic clone, Osg6B, was isolated, and its 5′-upstream region was found to regulate β-glucuronidase expression in the tapetum of transgenic tobacco. A set of 5′ deletions was also generated and a 1095 bp 5′ region was revealed to be necessary for activation of the Osg6B promoter in transgenic tobacco.",

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T1 - Molecular characterization of rice genes specifically expressed in the anther tapetum

AU - Tsuchiya, Tohru

AU - Toriyama, Kinya

AU - Ejiri, Shin ichiro

AU - Hinata, Kokichi

PY - 1994/12/1

Y1 - 1994/12/1

N2 - In situ localization of mRNA was carried out on two cDNAs (Osc4 and Osc6) that had been isolated from rice anthers at the microspore stage. The mRNA corresponding to each cDNA was shown to be localized only in the tapetal cells of the rice immature anthers, but not in the microspores or the mature pollen. The corresponding genomic clone, Osg6B, was isolated, and its 5′-upstream region was found to regulate β-glucuronidase expression in the tapetum of transgenic tobacco. A set of 5′ deletions was also generated and a 1095 bp 5′ region was revealed to be necessary for activation of the Osg6B promoter in transgenic tobacco.

AB - In situ localization of mRNA was carried out on two cDNAs (Osc4 and Osc6) that had been isolated from rice anthers at the microspore stage. The mRNA corresponding to each cDNA was shown to be localized only in the tapetal cells of the rice immature anthers, but not in the microspores or the mature pollen. The corresponding genomic clone, Osg6B, was isolated, and its 5′-upstream region was found to regulate β-glucuronidase expression in the tapetum of transgenic tobacco. A set of 5′ deletions was also generated and a 1095 bp 5′ region was revealed to be necessary for activation of the Osg6B promoter in transgenic tobacco.

KW - anther

KW - in situ hybridization

KW - promoter analysis

KW - rice

KW - tapetum

KW - transgenic tobacco

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