Molecular characterization of rice genes specifically expressed in the anther tapetum

Tohru Tsuchiya, Kinya Toriyama, Shin ichiro Ejiri, Kokichi Hinata

Research output: Contribution to journalArticlepeer-review

81 Citations (Scopus)

Abstract

In situ localization of mRNA was carried out on two cDNAs (Osc4 and Osc6) that had been isolated from rice anthers at the microspore stage. The mRNA corresponding to each cDNA was shown to be localized only in the tapetal cells of the rice immature anthers, but not in the microspores or the mature pollen. The corresponding genomic clone, Osg6B, was isolated, and its 5′-upstream region was found to regulate β-glucuronidase expression in the tapetum of transgenic tobacco. A set of 5′ deletions was also generated and a 1095 bp 5′ region was revealed to be necessary for activation of the Osg6B promoter in transgenic tobacco.

Original languageEnglish
Pages (from-to)1737-1746
Number of pages10
JournalPlant Molecular Biology
Volume26
Issue number6
DOIs
Publication statusPublished - 1994 Dec 1

Keywords

  • anther
  • in situ hybridization
  • promoter analysis
  • rice
  • tapetum
  • transgenic tobacco

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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