Molecular characterization of mutations induced by gamma irradiation in rice

Ryouhei Morita, Makoto Kusaba, Shuichi Iida, Hiroyasu Yamaguchi, Takeshi Nishio, Minoru Nishimura

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60 Citations (Scopus)

Abstract

In order to analyze mutations induced by gamma irradiation in higher plants, we irradiated rice with gamma rays and screened for mutations expressing phenotypes of glutinous endosperm (wx), chlorophyll b deficiency, endosperm protein deficiency, gibberellin-related dwarfism, and shortened plastochron - in order to clarify types of mutations. Nucleotide sequence analysis showed that the most frequent mutation induced by gamma rays was deletion, particularly small deletion. Of the 24 mutations, 15 were small deletions (1-16 bp), four were large deletions (9.4 -129.7 kbp), three were single-base substitutions, and two were inversions. Deletions 100 bp-8 kbp in length were not found, suggesting that gamma irradiation is unlikely to induce deletions of 100 bp to 8 kbp but is more likely to induce deletions between 1 and several ten bp or those of around 10 kbp or more. Based on the results, reverse genetics applications may be possible for gamma irradiation-induced deletions in rice by mismatch cleavage analysis used in Targeting Induced Local Lesions IN Genomes (TILLING) to detect small deletions and base substitutions or by using array comparative genomic hybridization (aCGH) to detect large deletions.

Original languageEnglish
Pages (from-to)361-370
Number of pages10
JournalGenes and Genetic Systems
Volume84
Issue number5
DOIs
Publication statusPublished - 2009 Dec 1

Keywords

  • Base substitution
  • Deletion
  • Gamma-irradiation
  • Inversion
  • Oryza sativa L.

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Morita, R., Kusaba, M., Iida, S., Yamaguchi, H., Nishio, T., & Nishimura, M. (2009). Molecular characterization of mutations induced by gamma irradiation in rice. Genes and Genetic Systems, 84(5), 361-370. https://doi.org/10.1266/ggs.84.361