Molecular characterization of LjABCG1, an ATP-binding cassette protein in Lotus japonicus

Akifumi Sugiyama, Shoju Fukuda, Kojiro Takanashi, Miki Yoshioka, Hirofumi Yoshioka, Yoshihiro Narusaka, Mari Narusaka, Mikiko Kojima, Hitoshi Sakakibara, Nobukazu Shitan, Shusei Sato, Satoshi Tabata, Masayoshi Kawaguchi, Kazufumi Yazaki

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    3 Citations (Scopus)

    Abstract

    LjABCG1, a full-size ABCG subfamily of ATP-binding cassette proteins of a model legume, Lotus japonicus, was reported as a gene highly expressed during the early stages of nodulation, but have not been characterized in detail. In this study we showed that the induction of LjABCG1 expression was remarkable by methyl jasmonate treatment, and reporter gene experiments indicated that LjABCG1 was strongly expressed in the nodule parenchyma and cell layers adjacent to the root vascular tissue toward the nodule. LjABCG1 was suggested to be localized at the plasma membrane based on the fractionation of microsomal membranes as well as separation via aqueous two-phase partitioning. The physiological functions of LjABCG1 in symbiosis and pathogenesis were analyzed in homologous and heterologous systems. LjABCG1 knock-down L. japonicus plants did not show clear phenotypic differences in nodule formation, and not in defense against Pseudomonas syringae, either. In contrast, when LjABCG1 was expressed in the Arabidopsispdr8-1 mutant, the penetration frequency of Phytophthora infestans, a potato late blight pathogen, was significantly reduced in LjABCG1/pdr8-1 than inpdr8-1 plants. This finding indicated that LjABCG1, at least partially, complemented the phenotype of pdr8 in Arabidopsis, suggesting the multiple roles of this protein in plant-microbe interactions.

    Original languageEnglish
    Article numbere0139127
    JournalPloS one
    Volume10
    Issue number9
    DOIs
    Publication statusPublished - 2015 Sep 29

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)
    • General

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  • Cite this

    Sugiyama, A., Fukuda, S., Takanashi, K., Yoshioka, M., Yoshioka, H., Narusaka, Y., Narusaka, M., Kojima, M., Sakakibara, H., Shitan, N., Sato, S., Tabata, S., Kawaguchi, M., & Yazaki, K. (2015). Molecular characterization of LjABCG1, an ATP-binding cassette protein in Lotus japonicus. PloS one, 10(9), [e0139127]. https://doi.org/10.1371/journal.pone.0139127