Molecular characterization of Cryptosporidium in native beef calves in central Vietnam

Sam Thi Nguyen, Yasuhiro Fukuda, Chika Tada, Rintaro Sato, Binh Duong, Duc Tan Nguyen, Yutaka Nakai

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The aims of this study were to investigate the prevalence of Cryptosporidium and to characterize the genotype distribution of Cryptosporidium isolates in native beef calves 2-6 months old in Dac Lac province, central Vietnam. The presence of Cryptosporidium oocysts was determined using the modified Ziehl-Neelsen staining method. The overall prevalence on the sample and herd levels were 18.9 % (44/232) and 50 % (20/40), respectively. Genotyping based on PCR and sequence analysis of the 18 S rRNA gene revealed occurrence of the two nonzoonotic species Cryptosporidium ryanae and Cryptosporidium bovis, with the former as a dominant species in the animals. The absence of the zoonotic species Cryptosporidium parvum in calves examined suggests that the native beef calves 2-6 months old in the study area are unlikely to contribute to human cryptosporidiosis transmission.

Original languageEnglish
Pages (from-to)1817-1820
Number of pages4
JournalParasitology Research
Volume111
Issue number4
DOIs
Publication statusPublished - 2012 Oct

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

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