Molecular Characterization of Adenosine 5′-monophosphate Deaminase-The Key Enzyme Responsible for the Umami Taste of Nori (Porphyra yezoensis Ueda, Rhodophyta)

Seiko Minami, Minoru Sato, Yoshihiro Shiraiwa, Koji Iwamoto

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The enzyme adenosine 5′-monophosphate deaminase (AMPD, EC 3. 5. 4. 6) catalyzes the conversion of adenosine 5′-monophosphate to inosine 5′-mononucleotide (IMP). IMP is generally known as the compound responsible for the umami taste of the edible red alga Porphyra yezoensis Ueda that is known in Japan as nori. Therefore, we suspect that AMPD plays a key role in providing a favorable nori taste. In this study, we undertake the molecular characterization of nori-derived AMPD. The nori AMPD protein has a molecular mass of 55 kDa as estimated from both gel filtration and sodium dodecyl sulfate polyacrylamide gel electrophoresis. The calculated molecular mass from the amino acid sequence deduced from cDNA is 57. 1 kDa. The isoelectric point is 5. 71. The coding region of AMPD consists of 1,566 bp encoding 522 amino acids and possesses a transmembrane domain and two N-glycosylation sites. The sequence identity of nori AMPD in human and yeast AMPDs was found to be less than 50% and 20% in DNA and amino acid sequences, respectively. Proline in the conserved motif of [SA]-[LIVM]-[NGS]-[STA]-D-D-P was found to be converted to glutamate. These results indicate that nori AMPD is a novel type of AMPD.

Original languageEnglish
Pages (from-to)1140-1147
Number of pages8
JournalMarine Biotechnology
Volume13
Issue number6
DOIs
Publication statusPublished - 2011 Dec 1

Keywords

  • 5′-AMP deaminase (AMPD)
  • 5′-inosinic acid (IMP)
  • Nori
  • Porphyra yezoensis
  • Umami

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

Fingerprint Dive into the research topics of 'Molecular Characterization of Adenosine 5′-monophosphate Deaminase-The Key Enzyme Responsible for the Umami Taste of Nori (Porphyra yezoensis Ueda, Rhodophyta)'. Together they form a unique fingerprint.

Cite this