Molecular Characterization of a Strain of Squash Leaf Curl China Virus from the Philippines

T. Kon, L. M. Dolores, N. B. Bajet, S. Hase, Hideki Takahashi, M. Ikegami

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

The complete nucleotide sequence of infectious cloned DNA components (A and B) of the causal agent of squash leaf curl disease in the Philippines was determined. DNA-A and DNA-B comprise 2739 and 2705 nucleotides, respectively; the common region is 174 bases in length. Five ORFs were found in DNA-A and two in DNA-B. Partial dimeric clones containing DNA-A and DNA-B, constructed in a binary vector and transformed into Agrobacterium tumefaciens, induced systemic infection in agro-inoculated pumpkin plants (Cucurbita moschata). The total DNA-A sequence was most closely related to that of Squash leaf curl China virus (SLCCNV) (88% identity), although the existence of B component of SLCCNV has not been reported. The deduced coat protein was like that of SLCCNV (98% amino acid sequence identity) and the Philippines virus has low sequence identity to Squash leaf curl virus (SLCV) and Squash mild leaf curl virus (SMLCV) (63 and 64% total nucleotide sequence identities, respectively). From these results, we propose that the Philippines virus be designated Squash leaf curl China virus-[Philippines] (SLCCNV-[PH]).

Original languageEnglish
Pages (from-to)535-539
Number of pages5
JournalJournal of Phytopathology
Volume151
Issue number10
DOIs
Publication statusPublished - 2003 Oct 1

Keywords

  • Agroinoculation
  • Begomovirus
  • Geminiviridae
  • Squash leaf curl virus

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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