Molecular Characterization of a Strain of Squash Leaf Curl China Virus from the Philippines

T. Kon, L. M. Dolores, N. B. Bajet, S. Hase, H. Takahashi, M. Ikegami

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35 Citations (Scopus)


The complete nucleotide sequence of infectious cloned DNA components (A and B) of the causal agent of squash leaf curl disease in the Philippines was determined. DNA-A and DNA-B comprise 2739 and 2705 nucleotides, respectively; the common region is 174 bases in length. Five ORFs were found in DNA-A and two in DNA-B. Partial dimeric clones containing DNA-A and DNA-B, constructed in a binary vector and transformed into Agrobacterium tumefaciens, induced systemic infection in agro-inoculated pumpkin plants (Cucurbita moschata). The total DNA-A sequence was most closely related to that of Squash leaf curl China virus (SLCCNV) (88% identity), although the existence of B component of SLCCNV has not been reported. The deduced coat protein was like that of SLCCNV (98% amino acid sequence identity) and the Philippines virus has low sequence identity to Squash leaf curl virus (SLCV) and Squash mild leaf curl virus (SMLCV) (63 and 64% total nucleotide sequence identities, respectively). From these results, we propose that the Philippines virus be designated Squash leaf curl China virus-[Philippines] (SLCCNV-[PH]).

Original languageEnglish
Pages (from-to)535-539
Number of pages5
JournalJournal of Phytopathology
Issue number10
Publication statusPublished - 2003 Oct


  • Agroinoculation
  • Begomovirus
  • Geminiviridae
  • Squash leaf curl virus

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Agronomy and Crop Science
  • Plant Science


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