TY - JOUR
T1 - Molecular characterization of a human monoclonal antibody to B antigen in ABO blood type
AU - Kaneko, Mika
AU - Kato, Yukinari
AU - Horiuchi, Hidekazu
AU - Osawa, Motoki
N1 - Funding Information:
We thank Dr E. Curiel-Quesada, Departamento de Bioquimica, Escuela Nacional de Ciencias Biologicas del I. P. N., and Dr D.L. Siegel, Department of Laboratory Medicine and Pathology, University of Pennsylvania, who kindly provided the sequences of their anti-ABH antibodies. This work was supported by a Grant-in-Aid for Scientific Research, 13770218, from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2003/3/3
Y1 - 2003/3/3
N2 - A human anti-B antibody of clone BT97 was obtained from a healthy individual of type A of the ABO blood group without immunization. Cloning was performed by means of heterohybridoma formation of cell fusion between human peripheral lymphocytes and mouse myeloma cells. The antibody selectively reacted with B-antigen in flow cytometry using red blood cells and enzyme-linked immunosorbent assay. The VH and VL genes of BT97 were derived from the germline genes of DP-47 and 3p.81A4, respectively, with a couple of somatic mutational events. Comparative analysis with other reported anti-A, B and H antibodies revealed that the amino acid sequence of the VH region was more homologous than that of the VL region. The sequence of BT97 showed complete identity with one anti-H natural antibody reported by Marks et al., with the exception of the CDR3 region. It is not known whether the homologies include the common properties of the natural antibodies; however, a particular germline gene potentially changes to anti-ABH antibodies. We think that this method is suitable for cDNA preparation of human monoclonal antibodies to blood group antigens and for sequence analysis.
AB - A human anti-B antibody of clone BT97 was obtained from a healthy individual of type A of the ABO blood group without immunization. Cloning was performed by means of heterohybridoma formation of cell fusion between human peripheral lymphocytes and mouse myeloma cells. The antibody selectively reacted with B-antigen in flow cytometry using red blood cells and enzyme-linked immunosorbent assay. The VH and VL genes of BT97 were derived from the germline genes of DP-47 and 3p.81A4, respectively, with a couple of somatic mutational events. Comparative analysis with other reported anti-A, B and H antibodies revealed that the amino acid sequence of the VH region was more homologous than that of the VL region. The sequence of BT97 showed complete identity with one anti-H natural antibody reported by Marks et al., with the exception of the CDR3 region. It is not known whether the homologies include the common properties of the natural antibodies; however, a particular germline gene potentially changes to anti-ABH antibodies. We think that this method is suitable for cDNA preparation of human monoclonal antibodies to blood group antigens and for sequence analysis.
KW - ABO histo-blood type
KW - Heterohybridoma
KW - Human monoclonal antibody
KW - Natural antibody
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U2 - 10.1016/S0165-2478(02)00294-8
DO - 10.1016/S0165-2478(02)00294-8
M3 - Article
C2 - 12600744
AN - SCOPUS:0037416790
VL - 86
SP - 45
EP - 51
JO - Immunology Letters
JF - Immunology Letters
SN - 0165-2478
IS - 1
ER -
