Molecular characterization of a gene encoding a photolyase from Streptomyces griseus

T. Kobayashi, M. Takao, A. Oikawa, A. Yasui

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

By using a synthetic DNA probe derived from an amino acid sequence in the most conserved region of three known photolyases (Escherichia coli, Anacystis nidulans and Saccharomyces cerevisiae), we isolated a DNA fragment containing two long open reading frames (ORFs) from a genomic DNA library of streptomyces griseus.One ORF encodes a polypeptide of 455 amino acids (Mr 50594), which exhibits substantial similarities with the other three photolyases. Photoreactivation-repair deficient E.coli cells could be converted into photoreactivatable ones by introduction of plasmids harboring this ORF, indicating that this is the photolyase gene of s.griseus. The deduced aa sequence of Streptomyces photolyase was most similar to that of E.coli. The putative DNA binding site as well as cofactor binding regions were proposed.

Original languageEnglish
Pages (from-to)4731-4744
Number of pages14
JournalNucleic acids research
Volume17
Issue number12
DOIs
Publication statusPublished - 1989 Jun 26

ASJC Scopus subject areas

  • Genetics

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