Molecular breeding of miraculin-accumulating tomatoes with suitable traits for cultivation in plant factories with artificial lightings and the optimization of cultivation methods

Many years have passed since the initial use of plant factories with artificial lighting (PFAL). However, while plant factories for various crops have been studied and developed, little has been done to develop crops best suited for plant factories. In this chapter, we present an example of developing a new medium-sized tomato of the indeterminate type, which accumulates miraculin, through crossing with a dwarf tomato parent to obtain a variety with suitable traits for plant factory cultivation. PFALs have the advantage of controlling all environmental factors for crop cultivation. This advantage makes it possible to set growing conditions that stably produce nutrient-valuable components and recombinant proteins. However, these growing conditions do not always coincide with what are generally considered good growing conditions. Thus, the optimal growing conditions for maximizing tomato fruit productivity and productivity of the target product, miraculin, are also presented using cross-bred lines grown under various light conditions.