Molecular and immunological characterization of Babesia gibsoni and Babesia microti heat shock protein-70

M. Alaa Terkawi, G. Aboge, H. Jia, Y. K. Goo, H. Ooka, J. Yamagishi, Y. Nishikawa, N. Yokoyama, I. Igarashi, S. I. Kawazu, K. Fujisaki, X. Xuan

    Research output: Contribution to journalArticlepeer-review

    18 Citations (Scopus)


    Serological immunoscreening was used to identify a gene encoding heat shock protein-70 from Babesia gibsoni (BgHSP-70) that showed high homology with HSP-70s from other apicomplexan parasites. This gene corresponded to a full-length cDNA containing an open reading frame of 1968 bp predicted to result in a 70-kDa mature protein consisting of 656 amino acids. Analysis of the expression levels of BgHSP-70 indicated elevated transcription from cultured parasites incubated at 40°C for 1 h, but not at 30°C. Interestingly, antiserum raised against recombinant BgHSP-70 protein reacted specifically not only with a 70-kDa protein of B. gibsoni but also with a corresponding native protein of B. microti (BmHSP-70), indicating the high degree of conservation of this protein. The BmHSP-70 gene was then isolated and characterized and the immunoprotective properties of recombinant BgHSP-70 (rBgHSP-70) and rBmHSP-70 were compared in vitro and in vivo. Both proteins had potent mitogenic effects on murine and canine mononuclear cells as evidenced by high proliferative responses and IFN-γ production after stimulation. Immunization regimes in BALB/c and C57BL/6 mice using rBgHSP-70 and rBmHSP-70 elicited high antibody levels, with concurrent significant reductions in peripheral parasitaemias. Taken together, these results emphasize the potential of HSP-70s as a molecular adjuvant vaccine.

    Original languageEnglish
    Pages (from-to)328-340
    Number of pages13
    JournalParasite Immunology
    Issue number6
    Publication statusPublished - 2009 Jun 1


    • Babesia gibsoni
    • Babesia microti
    • Heat shock protein-70
    • Immunization
    • Protection

    ASJC Scopus subject areas

    • Parasitology
    • Immunology


    Dive into the research topics of 'Molecular and immunological characterization of Babesia gibsoni and Babesia microti heat shock protein-70'. Together they form a unique fingerprint.

    Cite this