Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis

Chika Tateda, Kanako Watanabe, Tomonobu Kusano, Yoshihiro Takahashi

    Research output: Contribution to journalArticle

    49 Citations (Scopus)

    Abstract

    The voltage-dependent anion channel (VDAC), a major outer mitochondrial membrane protein, is thought to play an important role in energy production and apoptotic cell death in mammalian systems. However, the function of VDACs in plants is largely unknown. In order to determine the individual function of plant VDACs, molecular and genetic analysis was performed on four VDAC genes, VDAC1-VDAC4, found in Arabidopsis thaliana. VDAC1 and VDAC3 possess the eukaryotic mitochondrial porin signature (MPS) in their C-termini, while VDAC2 and VDAC4 do not. Localization analysis of VDAC-green fluorescent protein (GFP) fusions and their chimeric or mutated derivatives revealed that the MPS sequence is important for mitochondrial localization. Through the functional analysis of vdac knockout mutants due to T-DNA insertion, VDAC2 and VDAC4 which are expressed in the whole plant body are important for various physiological functions such as leaf development, the steady state of the mitochondrial membrane potential, and pollen development. Moreover, it was demonstrated that VDAC1 is not only necessary for normal growth but also important for disease resistance through regulation of hydrogen peroxide generation.

    Original languageEnglish
    Pages (from-to)4773-4785
    Number of pages13
    JournalJournal of experimental botany
    Volume62
    Issue number14
    DOIs
    Publication statusPublished - 2011 Oct

    Keywords

    • Arabidopsis thaliana
    • defence response
    • mitochondrial membrane potential
    • mitochondrial porin signature
    • pollen germination
    • voltage-dependent anion channel

    ASJC Scopus subject areas

    • Physiology
    • Plant Science

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