Modulation of endoplasmic reticulum (ER) stress-induced autophagy by C/EBP homologous protein (CHOP) and inositol-requiring enzyme 1α (IRE1α) in human colon cancer cells

Yosuke Shimodaira, Seiichi Takahashi, Yoshitaka Kinouchi, Katsuya Endo, Hisashi Shiga, Yoichi Kakuta, Masatake Kuroha, Tooru Shimosegawa

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

To explore the relationship between UPR and autophagy in intestinal epithelial cells, we investigated whether autophagy was induced by endoplasmic reticulum (ER) stress in colon cancer cell lines. We demonstrated that autophagy was induced by ER stress in HT29, SW480, and Caco-2 cells. In these cells, inositol-requiring enzyme1α (IRE1α) and C/EBP homologous protein (CHOP) were involved in the ER stress-autophagy pathway, and CHOP was a regulator of IRE1α protein expression. Our findings suggest that CHOP promotes IRE1α and autophagy especially in ER stress conditions. This study will provide important insights into the disclosure of the ER stress-autophagy pathway.

Original languageEnglish
Pages (from-to)524-533
Number of pages10
JournalBiochemical and biophysical research communications
Volume445
Issue number2
DOIs
Publication statusPublished - 2014 Mar 7

Keywords

  • Autophagy
  • CHOP
  • ER stress
  • IRE1α

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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