Modulating dynamics and function of nuclear actin with synthetic bicyclic peptides

Nanako Machida, Daisuke Takahashi, Yuya Ueno, Yoshihiro Nakama, Raphael J. Gubeli, Davide Bertoldo, Masahiko Harata

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    Actin exists in monomeric globular (G-) and polymerized filamentous (F-) forms and the dynamics of its polymerization/depolymerization are tightly regulated in both the cytoplasm and the nucleus. Various essential functions of nuclear actin have been identified including regulation of gene expression and involvement in the repair of DNA double-strand breaks (DSB). Small G-actin-binding molecules affect F-actin formation and can be utilized for analysis and manipulation of actin in living cells. However, these G-actin-binding molecules are obtained by extraction from natural sources or through complex chemical synthesis procedures, and therefore, the generation of their derivatives for analytical tools is underdeveloped. In addition, their effects on nuclear actin cannot be separately evaluated from those on cytoplasmic actin. Previously, we have generated synthetic bicyclic peptides, consisting of two macrocyclic rings, which bind to G-actin but not to F-actin. Here, we describe the introduction of these bicyclic peptides into living cells. Furthermore, by conjugation to a nuclear localization signal (NLS), the bicyclic peptides accumulated in the nucleus. The NLS-bicyclic peptides repress the formation of nuclear F-actin, and impair transcriptional regulation and DSB repair. These observations highlight a potential role for NLS-linked bicyclic peptides in the manipulation of dynamics and functions of nuclear actin.

    Original languageEnglish
    Pages (from-to)295-302
    Number of pages8
    JournalJournal of biochemistry
    Volume169
    Issue number3
    DOIs
    Publication statusPublished - 2021 Mar 1

    Keywords

    • actin polymerization
    • actin-binding molecule
    • bicyclic peptide
    • gene expression
    • nuclear actin

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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